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Albumin Analysis by Potentiometric Method

Albumin Analysis by Potentiometric Method. Fig 4. The cyclic voltammogram of albumin-modified Au wire electrode

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Albumin Analysis by Potentiometric Method

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  1. Albumin Analysis by Potentiometric Method

  2. Fig 4. The cyclic voltammogram of albumin-modified Au wire electrode WE: Albumin-modified Au wire, WE area: 0.0113 cm2, RE: Ag/AgCl(0.5M), CE: Pt, Electrolyte: 0.5 M KCl 50 ml(20 mM K4Fe(CN)6+20 mM K3Fe(CN)6), Potentiostat: CHI-405, Scan range: -0.2~0.6 V, scan rate: 0.05 V/sec

  3. Table Ⅲ: The relationship of peak current and albumin concentration

  4. #1 Process of preparing Au wire electrode Seal the electrode by epoxy adhesive excepts the sensing area(wait for a night) Wash by DI water for several times Polish the electrode surface by polishing paper (1500 mesh) Dry by N2(g) Clean the electrode by ultrasonic for 3 min. Dip into 1N HCl(aq)for 10min. Wash by DI water for several times wipe by delicate task wiper Dip into 1N NaOH(aq)for 10min.

  5. #1 Process of preparing Au wire electrode Dry by N2(g) *Dip in 10ml DI water for 20sec. for five times Dip into 1N HNO3(aq)for 10min. Dry by N2(g) Wash by DI water for several times Dip into 0.1ml albumin(aq)/0.5M KCl for a setting period. Dry by N2(g) Wash by DI water for several times Scan C-V (0ppm)

  6. #1 Process of preparing Au wire electrode Dry by N2(g) Wash by DI water for several times Scan C-V Dry by N2(g) Dip into 10ml DI water for 20sec. for five times Scan C-V Dry by N2(g) Dip into 50ml 6M urea for 1hr

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