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Colony-forming frequency (%)

si GFP. si GFP. si GFP. si FAK. si FAK. si FAK. 60. 100. 50. 80. 40. 60. Clonogenic efficiency (%). 30. 40. 20. 20. 10. 0. HT-25. RKO. 0. RKO. HT-25. C. HT-25 RKO. A. Adh. Susp. Adh. Susp. IB : FAK Paxillin poY118 GAPDH.

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Colony-forming frequency (%)

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  1. si GFP si GFP si GFP si FAK si FAK si FAK 60 100 50 80 40 60 Clonogenic efficiency (%) 30 40 20 20 10 0 HT-25 RKO 0 RKO HT-25 C HT-25 RKO A Adh. Susp. Adh. Susp. IB: FAK PaxillinpoY118 GAPDH siFAK: -- + -- + -- + -- + D B 1.75 1.50 1.25 Colony-forming frequency (%) 1.00 Normalized colony formation 0.75 0.50 0.25 0 HT-25 RKO

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