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IGEM TEAM EPF-LAUSANNE

IGEM TEAM EPF-LAUSANNE. WHAT IS IGEM?. I nternational G enetically E ngineered M achine Having received a kit of biological parts from the iGEM Registry, we start to build our biological systems over the summer.

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IGEM TEAM EPF-LAUSANNE

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  1. IGEM TEAM EPF-LAUSANNE

  2. WHAT IS IGEM? International Genetically Engineered Machine Having received a kit of biological parts from the iGEM Registry, we start to build our biological systems over the summer. The perspective of the Jamboree in November at MIT and the project design is exceptionally motivating, in addition to the fact that this is a good learning experience.

  3. OUR TEAM & OUR SCHOOL

  4. THE PROJECT: WET LAB PART

  5. Cloning Parts:  using the BioBrick protocol of Ginkgo Bioworks THE PROJECT: WET LAB PART

  6. THE PROJECT: MAKING A PART

  7. THE PROJECT: WET LAB PART The BphP / PpsR system

  8. THE PROJECT: MODELING PART-AIM Goal: Enhance the binary effect of the light switch • Molecular dynamics (MD) simulation of the photosensitive protein domain on computer for two protein states: • The light activated state • The ground state (protein without light excitation) Study of both state stability (statistical analysis) Determination of amino acids that could be mutated to enhance stability of light activated state Mutation of the amino acids and test the switch in vivo

  9. THE PROJECT: MODELING PART-PRELIMINARY RESULTS • First nanosecond MD simulation:

  10. THE PROJECT: APPLICATIONS Merck-Serono interests: using light to induce gene transcription in mammaliancells

  11. Our Mentors: Sebastian MaerklLaboratory of Biological Network Characterization (STI/SV) Bart DeplanckeLaboratory of Systems Biology and Genetics Matteo Dal PeraroLaboratory for Biomolecular Modeling Felix Naef Computational systems biology THANK YOU!

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