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Virginia iGEM

Explore DNA assembly techniques through hands-on activities and in-depth reading about enzymatic assembly. Learn about restriction enzyme digests, ligation reactions, and Gibson assembly in a fun and educational environment.

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Virginia iGEM

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  1. VirginiaiGEM Workshop #3 High School Education Series

  2. Workshop Plan • Lesson: Restriction Enzyme Digest, Ligation Reaction, & Gibson Assembly • Activity: Make Your Own • BioBrick (Exploring the • Parts Registry) • Follow-up Reading: • Enzymatic Assembly of DNA • Molecules up to Several Hundred Kilobases, • Gibson et. al. VirginiaiGEM Workshop #3 High School Education Series

  3. Restriction Enzyme Digest Restriction Enzyme Digest: http://igem.org/Videos/Restriction_Digest VirginiaiGEM Workshop #3 High School Education Series

  4. Calf Intestinal Alkaline Phosphatase VirginiaiGEM Workshop #3 High School Education Series

  5. Ligation Reaction Ligation Reacion: http://igem.org/Videos/Ligating_Your_Parts VirginiaiGEM Workshop #3 High School Education Series

  6. Gibson Assembly VirginiaiGEM Workshop #3 High School Education Series

  7. Activity: Make Your Own BioBrick VirginiaiGEM Workshop #3 High School Education Series

  8. Follow-up Reading: Enzymatic Assembly of DNA Molecules up to Several Hundred Kilobases, Gibson et. al. VirginiaiGEM Workshop #3 High School Education Series

  9. Any Questions? VirginiaiGEM Workshop #3 High School Education Series

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