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It’s A Wonderful Phage Life

It’s A Wonderful Phage Life. Danielle Hoxworth. Who is this Phage?. Mr. Tibbs Comes from the movie The Heat of the Night. It is also the name of my stuffed microwavable owl, chosen by my friends. Where Did H e C ome F rom?.

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It’s A Wonderful Phage Life

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  1. It’s A Wonderful Phage Life Danielle Hoxworth

  2. Who is this Phage? • Mr. Tibbs • Comes from the movie The Heat of the Night. It is also the name of my stuffed microwavable owl, chosen by my friends.

  3. Where Did He Come From? • Found the soil on the banks of the river down by the falls in River Falls, Wisconsin. It had rained that morning, it was slightly muddy and there was plants and grass growing by the water, thought it was a good place.

  4. A Visual

  5. On the outside • Size: 2 mm without the halo, 3 mm with halo. • Appearance: There are halos around most of the plaques, with the plaques varying in size. The halos are sort of a cloudy color, while the plaques are clear.

  6. On the Inside • Titer of HTL was 5.3 x 106 • DNA Isolation: • Concentration of 0.074 µg/µL. • Yield of 7.4 µg • The A260/280 ratio was 1.591. That ratio means that the DNA within Mr. Tibbs is mostly pure.

  7. Restriction Digest Results

  8. DNA Gel Results Explained • The picture shown in Slide number 7 is the results of the 1st and only DNA gel that I ran during the course of the semester. It looked very similar to many of the other gel photos from the other students in the lab, this is because the enzymes shown in each of the lanes did not spread as well or as far as they should have, which is why many of the lines are very short.

  9. Any Similarities? • There are a few similarities: • Different sized plaques throughout experiment. • Saldago. • Saldago was one of the phages that have been submitted to the database after using Arthrobacter bacteria in the experiment.

  10. Anything Else? Results from beginning of semester to now. -progression and changes

  11. The Interesting Life of a Phage • The Latin meaning of the word “virus” actually means “poison”. This is actually really cool because thinking about it, viruses can indeed “poison” or damage the host that they leeched onto. • It is astounding that phages have the capability to “live” through another organism. They cannot live on their own but they have the capability to thrive through something else, which in my opinion would take so much more work.

  12. More Into Their Interesting Life • Phages date back to the beginning of cellular life. As old as they are, it seems that science is still having a constant struggle pinpointing exactly what a virus is, in terms of being alive or not alive. • The most fascinating fact to me, is that viruses have the capability to sometimes returned back to the “life” they had within the host after being destroyed. For something that is considered to be not alive, it almost can bring itself back to life. • All of these facts taken from the “Are Viruses Alive?” article written by Luis P. Villarreal. Scientific American, Inc. December 2004.

  13. The Parts That Are Missing… • I was not able to include a 2nd set of DNA gel results OR the results of the electron microscope as I was absent during those times and did not get to participate in those lab sessions.

  14. Was My Phage Unique? • It was very difficult for me to make a solid decision as to whether my phage was unique, or if it looked like others from within the class. I came to the conclusion, that my 1st DNA gel results photo looked too similar to other students’ to be unique. I believe this is partly due to the 1st DNA gel results all looking very similar, but I also do not believe I have a unique phage.

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