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Ca 2+ release mechanisms in cardiac & skeletal muscle

Ca 2+ release mechanisms in cardiac & skeletal muscle. OBJECTIVES Methodology: Chimeric proteins FRET Differences in Ca 2+ release between cardiac and skeletal muscle . INTRODUCTION. Ca 2+ channel characteristics . Voltage sensitive Ca 2+ channel molecular structure.

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Ca 2+ release mechanisms in cardiac & skeletal muscle

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  1. Ca2+ release mechanisms in cardiac & skeletal muscle OBJECTIVES Methodology: Chimeric proteins FRET Differences in Ca2+ release between cardiac and skeletal muscle

  2. INTRODUCTION

  3. Ca2+ channel characteristics

  4. Voltage sensitive Ca2+ channel molecular structure

  5. Mammalian Ca2+ channel genes

  6. SR Ca2+ release channel – Ryanodine receptor (RyR)

  7. RyR molecular structure

  8. Mammalian ligand-gated Ca2+ channel genes

  9. DHPR-RyR molecular arrangment

  10. Ca2+ release in skeletal muscle

  11. Ca2+ release in cardiac muscle

  12. DISCUSSION

  13. Cardiac sequence Skeletal muscle sequence pCac6 (Sk) 1.5 nA pCARD1 15 nA 10 nA

  14. CELL SHORTENING Cardiac sequence Skeletal muscle sequence pCac6 (Sk) pCARD1 Ca2+ + 0 / Cd2+

  15. I II III IV II-III LOOP

  16. Serine687 to alanine (S687A) Serine – PKA (only in Sk)

  17. I II III IV II-III LOOP

  18. Region with RyR-2 cDNA injection Dyspedic myotubes Ca2+ transient during spontaneous contractions Normal myotubes Dyspedic myotubes with RyR-2

  19. Normal myotubes Dyspedic myotubes with RyR-2

  20. Ca2+ current & [Ca2+]i RyR-2 expressing Dyspedic myotubes Ca2+ current RyR-2 expressing Dyspedic myotubes

  21. Ca2+ current CSk3 expressing Dyspedic myotubes Ca2+ transient Dyspedic myotubes Electrically evoked Spontaneous Failure (electrical)

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