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SHOTARO YAMAGUCHI and MASAAKI YOKOE Applied And Environmental Microbiology 66 (2000) 3337-3343

A Novel Protein-Deamidating Enzyme from Chryseobacterium proteolyticum sp. nov., a Newly Isolated Bacterium from Soil. SHOTARO YAMAGUCHI and MASAAKI YOKOE Applied And Environmental Microbiology 66 (2000) 3337-3343 報告學生：莊涵為. Protien-Deamidating Enzyme. 能使 protien 去胺基 在工業上被應用來使 protien 乳化或起泡.

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SHOTARO YAMAGUCHI and MASAAKI YOKOE Applied And Environmental Microbiology 66 (2000) 3337-3343

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  1. A Novel Protein-Deamidating Enzyme from Chryseobacterium proteolyticum sp. nov., a Newly Isolated Bacterium from Soil SHOTARO YAMAGUCHI and MASAAKI YOKOE Applied And Environmental Microbiology 66 (2000) 3337-3343 報告學生：莊涵為

  2. Protien-Deamidating Enzyme • 能使protien去胺基 • 在工業上被應用來使protien乳化或起泡

  3. 實驗流程 採樣 菌株培養與分離 酵素測定 菌株分類

  4. 320 soil samples, collected from natural environments • grasslands,gardens, crop field, livestock farms, riversides, forests, and dumping grounds • Japan

  5. 實驗流程 採樣 菌株培養與分離 酵素測定 菌株分類

  6. A medium：0.1% carboxybenzoxy (Cbz)-Gln-Gly, 0.5% glucose, 0.02% KH2PO4, 0.02% MgSO4．7H2O, 0.01% NaCl, 0.002% CaCl2, 0.0002% FeSO4．7H2O, 0.0005% NaMO4．2H2O, 0.0005% NaWO4．4H2O, 0.0005% MnSO4．4H2O, 0.01% CuSO4．5H2O • Isolated by plating or streaking of the second culture onto LBA or PDA • Luria bertani agar(LBA)：Bacteria • Potato dextrose agar(PDA)：Fungi • B medium：0.5% lactose, 1.0% peptone, 0.17% NaHPO4．H2O, 0.025% KH2PO4, 0.025% MgSO4．7H2O, 0.005% FeSO4．7H2O

  7. 實驗流程 採樣 菌株培養與分離 酵素測定 菌株分類

  8. Substrate solution containing: Cbz-Gln-Gly or caseins sodium phosphate buffer enzyme solution • Released ammonia was determined by an NADH-glutamate dehydrogenase method and phenol method • Two strains showing higher protein-deamidating activity

  9. 實驗流程 採樣 菌株培養與分離 酵素測定 新菌株分類

  10. Phenotypic characteristics • 16S RNA gene sequencing and analysis

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