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MICROARRAYS

MICROARRAYS. Microarray. Tool for studying how large numbers of genes interact with each other Offers insight on how a cell’s regulatory networks control vast batteries of genes simultaneously. http://genome.ornl.gov. Review. Lipids Carbohydrates Amino Acids Nucleic Acids. Central Dogma.

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MICROARRAYS

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  1. MICROARRAYS

  2. Microarray • Tool for studying how large numbers of genes interact with each other • Offers insight on how a cell’s regulatory networks control vast batteries of genes simultaneously

  3. http://genome.ornl.gov

  4. Review • Lipids • Carbohydrates • Amino Acids • Nucleic Acids

  5. Central Dogma

  6. Central Dogma

  7. Properties of DNA

  8. Microarray Process • Extract mRNA from samples • Convert to cDNA • Add fluorescent label to cDNA • Amplify via PCR • Hybridize to microarray • Wash

  9. mRNA extraction cDNA conversion PCR Amplification photolithography PCR amplification test tag

  10. Microarray Detail

  11. Microarray Detail

  12. Strengths • Runs multiple tests in tandem • Automated • Comprehensive • Speed

  13. Limitations • Problems with hybridization • Need for Strict experimental guidelines • Complex relationship between mRNA -> Protein • Validation

  14. Applications • Pharmaceutical • Biomedical Research • Clinical

  15. Profile of Microarray Today (c)2001 Adapted from ABRF Microarray Research Group Study

  16. END Q&A

  17. References

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