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Identification of IR-responsive miRNAs and their target genes in MCF7 cells

This study identifies and characterizes miRNAs that are responsive to ionizing radiation (IR) treatment in MCF7 breast cancer cells. The miR-770-5p is shown to induce apoptosis by down-regulating PBK gene expression in HCT116 colon carcinoma cells. The study also provides a list of putative target genes regulated by miR-770-5p.

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Identification of IR-responsive miRNAs and their target genes in MCF7 cells

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  1. IR 6 Gy IR 6 Gy IR 6 Gy IR 6 Gy 2 8 24 (h) 2 8 24 (h) 2 8 24 (h) 2 8 24 (h) hsa-miR-762 hsa-miR-197 hsa-miR-1287 hsa-miR-132 hsa-miR-188-5p hsa-miR-1228 hsa-miR-425* hsa-miR-1234 hsa-miR-1181 hsa-miR-99b* hsa-miR-614 hsa-miR-770-5p hsa-miR-10b* hsa-miR-874 hsa-miR-572 hsa-miR-1225-3p hsa-miR-33a hsa-miR-28-5p hsa-miR-34a* hsa-miR-500a hsa-miR-625 hsa-miR-550a* hsa-miR-1307 hsa-miR-191* hsa-miR-221 hsa-miR-30e* hsa-miR-338-3p hsa-miR-205 hsa-miR-141* hsa-miR-421 hsa-miR-532-5p hsa-miR-193a-5p hsa-miR-95 hsa-miR-744 hsa-miR-574-3p hsa-miR-483-5p hsa-miR-362-3p hsa-miR-342-5p hsa-miR-769-5p hsa-miR-30a* hsa-miR-362-5p hsa-miR-181c hsa-miR-431* hsa-miR-152 hsa-miR-340* hsa-miR-2276 hsa-miR-135b hsa-miR-206 hsa-miR-432 hsa-miR-371-5p hsa-miR-484 hsa-miR-1274a_v16.0 hsa-miR-193b hsa-miR-21* hsa-miR-450a hsa-miR-1246 hsa-miR-320d hsa-miR-320c hsa-miR-181a hsa-miR-34a hsa-miR-660 hsa-miR-196a hsa-miR-99b hsa-let-7b hsa-miR-320b hsa-miR-141 hsa-miR-25 hsa-miR-151-3p hsa-let-7d hsa-miR-1308_v15.0 hsa-miR-590-5p hsa-miR-324-5p hsa-miR-320a hsa-miR-1260 hsa-miR-19a hsa-miR-17 hsa-miR-1914* hsa-miR-331-3p hsa-miR-374b hsa-miR-17* hsa-miR-140-3p hsa-miR-652 hsa-miR-125b hsa-miR-29b hsa-miR-181b hsa-let-7e hsa-miR-92a hsa-miR-148b hsa-miR-1974_v14.0 hsa-miR-15b hsa-miR-342-3p hsa-miR-20a hsa-miR-16 hsa-miR-1288 hsa-miR-1280 hsa-miR-21 hsa-miR-106b hsa-miR-29a hsa-miR-19b hsa-miR-93 hsa-miR-1975_v15.0 hsa-miR-99a hsa-miR-30b hsa-let-7i hsa-miR-195 hsa-miR-126 hsa-miR-375 hsa-miR-22 hsa-miR-30d hsa-miR-200c hsa-miR-27a hsa-miR-1202 hsa-miR-185 hsa-miR-340 hsa-miR-151-5p hsa-miR-18a hsa-miR-16-2* hsa-miR-30a hsa-miR-301a hsa-let-7g hsa-miR-24 hsa-miR-130b hsa-miR-1977_v14.0 hsa-let-7a hsa-miR-96 hsa-miR-27b hsa-miR-429 hsa-miR-200b hsa-miR-128 hsa-miR-575 hsa-miR-103a hsa-let-7f hsa-miR-425 hsa-miR-18b hsa-miR-7 hsa-miR-1290 hsa-miR-374a hsa-miR-1973 hsa-miR-30e hsa-miR-513b hsa-miR-378 hsa-miR-34b* hsa-miR-29c hsa-miR-892b hsa-miR-15b* hsa-miR-148a hsa-miR-203 hsa-miR-15a hsa-miR-101 hsa-miR-335 hsa-miR-365 hsa-miR-629 hsa-miR-98 hsa-miR-183* hsa-miR-622 hsa-miR-106b* hsa-miR-503 hsa-let-7a* hsa-miR-615-3p hsa-miR-200b* hsa-miR-200c* hsa-miR-940 hsa-miR-210 hsa-miR-19b-1* hsa-miR-93* hsa-miR-501-5p hsa-miR-638 hsa-miR-150* hsa-let-7d* hsa-miR-1183 hsa-miR-1226* hsa-miR-1238 hsa-miR-190b hsa-miR-30d* hsa-miR-33b* hsa-miR-370 hsa-miR-586 hsa-miR-134 hsa-miR-1915 hsa-miR-181d hsa-miR-1207-5p hsa-miR-1268 hsa-miR-720 hsa-miR-7-1* hsa-miR-140-5p hsa-miR-181a* hsa-miR-513a-5p hsa-miR-1225-5p hsa-miR-574-5p hsa-miR-183 hsa-miR-1224-5p hsa-miR-301b hsa-miR-1275 hsa-miR-219-5p hsa-miR-1274b_v16.0 hsa-miR-497 hsa-miR-361-5p hsa-miR-125a-3p hsa-miR-505 hsa-miR-193a-3p hsa-miR-125a-5p hsa-miR-939 hsa-miR-423-5p -6.5 5.5 (log2) IR 6 Gy 2 8 24 (h) hsa-miR-107 hsa-miR-182 hsa-miR-26b hsa-let-7c hsa-miR-454 hsa-miR-23b hsa-miR-20b hsa-miR-424 hsa-miR-26a hsa-miR-200a hsa-miR-23a hsa-miR-186 hsa-miR-30c hsa-miR-494 hsa-miR-1305 hsa-miR-212 hsa-miR-100 hsa-miR-1979_v15.0 hsa-miR-487b hsa-miR-335* hsa-miR-324-3p hsa-miR-32 hsa-miR-513c hsa-miR-196b hsa-miR-338-5p hsa-miR-1826_v15.0 Supplementary Figure S1. Extended datasets of 229 IR-responsive miRNAs in MCF7 cells.

  2. a b * *** miR- HCT116 HCT116 HCT116 Relative miR-770-5p / 5S rRNA Relative PBK mRNA / Actin mRNA 770-5p 770-5p miR- miR- Con Con Actin Cleaved PARP PBK Supplementary Figure S2. Overexpression of miR-770-5p induces apoptosis via down-regulation of PBK in HCT116 human colon carcinoma cells. (a) After transfection of miR-Con or miR-770-5p in HCT116 cells, the relative levels of miR-770-5p and PBK mRNA were confirmed by qRT-PCR. (b) Levels of PBK protein and cleaved PARP were observed in miR-Con and miR-770-5p transfected HCT116 cells. Actin was used as a loading control. 770-5p Con 89 KDa 40 KDa 43 KDa

  3. Supplementary Table S1. List of 225 mRNAs which were repressed by IR

  4. Supplementary Table S2. List of 288 putative targets of miR-770-5p predicted based on miRSVR (< -0.75) from www.microrna.org website

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