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ClearH 2 O. Bilsky Laboratory University of New England August 6, 2012. Duration Assessment of DietGel 31M & HydroGel. Protocol. 4 week old male C57BL/6J mice (Jackson Laboratory) were received into the animal facility on June 28 th , 2012
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ClearH2O Bilsky Laboratory University of New England August 6, 2012 Duration Assessment of DietGel 31M & HydroGel
Protocol • 4 week old male C57BL/6J mice (Jackson Laboratory) were received into the animal facility on June 28th, 2012 • Mice were marked, weighed, and randomly housed 4 per cage into the three treatment groups: • Control (n=12) – Standard food and water • DietGel 31M (n=12) – DietGel 31M and standard water • HydroGel (n=12) – Standard food and HydroGel • Mouse were kept in the vivarium under standard housing conditions (temperature 22 ± 2°C and relative humidity between 55-60%) • Bodyweights were measured once a day to the nearest 0.05 gram at 8 AM for 31 days • On days 14 and 21mice were inspected by blinded laboratory personnel for overall health (e.g. coat and typical behavior) • On day 21 mice were visually inspected for tooth length
DATA: 14 Days Researcher: Alex Colville and Denise Giuvelis • Data shows the average bodyweight of mice on DietGel 31M versus control for 14 days and the average bodyweight of mice on HydroGel versus control for 14 days • A 2 way ANOVA revealed no statistical significance between the three treatments through the 14 days
DATA: 31 Days Researcher: Alex Colville and Denise Giuvelis Dates: 6/28-7/28/12 • Data shows the average bodyweight of both DietGel 31M and HydroGel versus control treatments for the entire 31 day study • *, **, *** denotes significance after a 2-way ANOVA with p < 0.05, 0.01, and 0.001 respectively
Conclusions: General Health • Blinded observation revealed no difference in appearance of the coat of the mice or any changes in behavior due to the varying treatment groups • Due to the hard rodent chow diet being replaced by a soft gel diet, tooth length was monitored • Tooth length was observed on day 21 and it was determined by a blinded observer that there were no differences between treatment groups