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Protein Synthesis Lab

Protein Synthesis Lab. Click Here to Begin Your Lab. Background.

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Protein Synthesis Lab

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  1. Protein Synthesis Lab Click Here to Begin Your Lab

  2. Background • Welcome to the CELL. Many process occur regularly that keep the CELL alive. Of these processes, one of the most important is a process called “Protein Synthesis.” It is this process that uses the information stored in DNA to create the CELL’S proteins. Click here to continue

  3. Warm-up Directions: On your sheet of paper, match each definition to the correct term • DNA • mRNA • codon • tRNA • Ribosome • Brings the amino acids to the ribosome • Assembles the protein by combining amino acids • Stores the information on how to make the various proteins of the body. • Is a copy of a gene that can leave the nucleus later to be read by a ribosome. • Equals 3 bases, also equals 1 amino acid Click here to continue

  4. The first step of Protein synthesis is called Transcription. Click on the organelle where transcription takes place in eukaryotic cells Mitochondria Mitochondria Rough E.R. Golgi Apparatus Smooth E.R. Nucleus Ribosomes

  5. Step 1: Transcription • Transcription is the first step of protein synthesis. This step takes place in the nucleus of eukaryotic cells. Segments of DNA called genes store the information on the proper order of amino acids to construct the cells proteins. Click on one of the chromosomes to see what genes they contain. Once you have finished with all 3 chromosomes, click here to answer the final lab questions. Chromosome 2 Chromosome 3 Chromosome 1

  6. Chromosome 1 • DNA is too valuable to allow it to leave the nucleus, so the cell copies it into the form of mRNA. Messenger RNA can then take this information out of the nucleus to the ribosomes to make the proteins. • Directions: You need to transcribe the DNA message below into the form of mRNA on your paper. Also write down what Chromosome you are working on. (Click here to review Base Pairing Rules) GCGCGCGTACAGGAAAGCCACAAGTTGTGATAGCGGGCGCATATTATCCTGCATCCGGTTTC Once you are done with transcription Click here to move to translation

  7. Chromosome 2 • DNA is too valuable to allow it to leave the nucleus, so the cell copies it into the form of mRNA. Messenger RNA can then take this information out of the nucleus to the ribosomes to make the proteins. • Directions: You need to transcribe the DNA message below into the form of mRNA on your paper. Also write down what Chromosome you are working on. (Click here to review Base Pairing Rules) CCGGAATCTACTAGTATTTCTAGGGTCTTACGAAACTCCGTCCCGTCATTCGTGCTATCCGA Once you are done with transcription Click here to move to translation

  8. Chromosome 3 • DNA is too valuable to allow it to leave the nucleus, so the cell copies it into the form of mRNA. Messenger RNA can then take this information out of the nucleus to the ribosomes to make the proteins. • Directions: You need to transcribe the DNA message below into the form of mRNA on your paper. Also write down what Chromosome you are working on.(Click here to review Base Pairing Rules) CTGCGCAACCTACCCTAAACTCGACTTTCATAGGAAAGACTTTCACATCGCCAGCATCC Once you are done with transcription Click here to move to translation

  9. Step 2: Translation • Translation is the second step in protein synthesis. Here, the mRNA is read by the ribosome by matching up codons to amino acids. • Directions: Use your mRNA and click on the codons to see what the amino acids are. Write down the amino acids on your paper. Click here to begin Translation

  10. Messenger RNA now leaves the nucleus. To begin translation click on the organelle that reads the mRNA and makes the protein. Mitochondria Mitochondria Rough E.R. Golgi Apparatus Smooth E.R. Nucleus Ribosomes

  11. Directions: Below are mRNA codons. Using your transcribed gene from the first part of the lab, click on the various codons to see what the amino acids are for each. Write the amino acids down in the proper order until you come to the stop codon. The amino acids in this lab are represented by words and linked together to make sentences (proteins). (Note: some codons may be used more than once). Once you have finished putting your protein together, click here. GUC UGG CUC AAA CAU CGA UAC UAU UGU GCA GCG UGA GAA CGU CGC UCG ACU AUU UCC GCU AAU UCU UAG ACA GUG GAG AGG UUU CGG GCC CUA AAG AGU GUU ACC AGA CCG CCA GAU UCA AUG AUC CCC GGA CUG GUA UUA AGC UUC ACG GAC GGU CCU AUA CAG CAA AAC UUG CAC GGG GGC UGC CUU UAA

  12. YOU

  13. WHAT

  14. FIND

  15. DO

  16. NOT

  17. BECOME

  18. EVEN

  19. I

  20. ACT

  21. COME

  22. FORWARD

  23. BUT

  24. STOP CODON THIS IS THE END OF YOUR PROTEIN (SENTENCE)

  25. SHINE

  26. STARS

  27. ON

  28. TO

  29. THEIR

  30. LET

  31. WHEN

  32. HAPPINESS

  33. SINGULAR

  34. MORE

  35. COURAGE

  36. HABIT

  37. IN

  38. PREPARE

  39. YOUR

  40. THEM

  41. STOP This is NOT a stop codon. This is a word in the sentence.

  42. THE

  43. EXCELLENCE

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