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B2 repeat

B2 repeat. PGN. pA. 3’ LTR. SA. enh. = Trap-specific nested primers = B2-specific primer = Suppression sequence. B2 repeat. B2 repeat. Gentile et al., Supplementary Figure 2

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B2 repeat

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  1. B2 repeat PGN pA 3’ LTR SA enh = Trap-specific nested primers = B2-specific primer = Suppression sequence B2 repeat B2 repeat Gentile et al., Supplementary Figure 2 RAN-PCR procedure for mapping trap integration sites. Specific amplification of genomic regions comprised between a B2 repeat and an integrated trap occurs by means of a B2-specific primer and two nested primers designed on the trap cassette, as indicated. A Suppression sequence added 5’ to the B2 primer avoids aspecific B2-B2 amplification by promoting the formation of panhandle-like structures which cannot be amplified, as illustrated in the lower part of the figure.

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