1 / 22

SURVEY OF BIOCHEMISTRY Nucleic Acids

SURVEY OF BIOCHEMISTRY Nucleic Acids. What are nucleic acids?. Building blocks of genetic material that a cell uses to direct cellular functions and heredity 3 Components: Sugar Moiety. Ribose (in RNA). Deoxyribose (in DNA). Ring Numbering in Ribose. 5’. 4’. 1’.

gusty
Download Presentation

SURVEY OF BIOCHEMISTRY Nucleic Acids

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. SURVEY OF BIOCHEMISTRYNucleic Acids CHEM 3511 - Summer 2008

  2. What are nucleic acids? • Building blocks of genetic material that a cell uses to direct cellular functions and heredity • 3 Components: Sugar Moiety Ribose (in RNA) Deoxyribose (in DNA) CHEM 3511 - Summer 2008

  3. Ring Numbering in Ribose 5’ 4’ 1’ DNA lacks the2’-OH group 3’ 2’ CHEM 3511 - Summer 2008

  4. What are nucleic acids? • Building blocks of genetic material that a cell uses to direct cellular functions and heredity • 3 Components: Phosphate Moiety Phosphate Group Phosphoric Acid CHEM 3511 - Summer 2008

  5. What are nucleic acids? • Building blocks of genetic material that a cell uses to direct cellular functions and heredity • 3 Components: 5 Nitrogenous Bases Guanine G PURINES Adenine A CHEM 3511 - Summer 2008

  6. What are nucleic acids? • Building blocks of genetic material that a cell uses to direct cellular functions and heredity • 3 Components: 5 Nitrogenous Bases Cytosine C Thymine T Uracil U PYRIMIDINES CHEM 3511 - Summer 2008

  7. Ring Numbering in Bases Adenine A 6 7 5 1 8 2 4 9 3 CHEM 3511 - Summer 2008

  8. Ring Numbering in Bases Cytosine C 4 5 3 2 6 1 CHEM 3511 - Summer 2008

  9. Nomenclature Base Nucleoside Nucleotide Adenine Adenosine Adenosine A Monophosphate Guanine Guanosine Guanosine G Monophosphate Etc. Study Table 3-1 for more details… CHEM 3511 - Summer 2008

  10. How do nucleotides link? CHEM 3511 - Summer 2008

  11. Base Pairing Carbon Nitrogen Oxygen Hydrogen CHEM 3511 - Summer 2008

  12. Base Pairing Carbon Nitrogen Oxygen Hydrogen CHEM 3511 - Summer 2008

  13. How Does RNA Differ from DNA? • Ribose vs. Deoxyribose • Bases and Base Pairing • T in DNA vs. U in RNA • A-T in DNA vs. A-U in RNA • 3D Structure • Role CHEM 3511 - Summer 2008

  14. Protein Data Bank http://www.rcsb.org/pdb/home/home.do CHEM 3511 - Summer 2008

  15. Protein Data Bank • Download PDB file to Desktop • Use Freeware to View Contents • RasMol • PyMol • Swiss PDB Viewer • Protein Explorer CHEM 3511 - Summer 2008

  16. Software for Viewing Structures • Rasmol • http://www.rasmol.org • Pymol • http://pymol.sourceforge.net • SwissPDB Viewer • http://www.expasy.ch/spdbv/ • Protein Explorer • http://www.umass.edu/microbio/chime/pe_beta/pe/protexpl/frntdoo2.htm CHEM 3511 - Summer 2008

  17. DNA Double Helix CHEM 3511 - Summer 2008

  18. DNA Structural Features • Handedness • Anti-parallel strands • Major and minor grooves CHEM 3511 - Summer 2008

  19. Different Conformations of DNA CHEM 3511 - Summer 2008

  20. DNA has incredible flexibility! Catabolite-ActivatorProtein (CAP) + DNA Yale (1991) CHEM 3511 - Summer 2008

  21. DNA has incredible flexibility! DNA Octahedron Ned SeemanNYU International Society for Nanoscale Science, Computation, and Engineering CHEM 3511 - Summer 2008

  22. More on Friday… • Limitations to DNA Flexibility • Supercoiling and Topoisomerases • RNA Structure • Nucleic Acid Function • Recombinant DNA Technology • Potentially PRS questions… CHEM 3511 - Summer 2008

More Related