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MICROBIOLOGY PRACTICAL

MICROBIOLOGY PRACTICAL. GI BLOCK; 2013. Objectives:. Understand the use of viral serological studies for the diagnosis of hepatitis A , B & C infections. To know measures to prevent hepatitis A & B infections. To know the viral serological tests used to screen blood donors.

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MICROBIOLOGY PRACTICAL

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  1. MICROBIOLOGYPRACTICAL GI BLOCK; 2013

  2. Objectives: • Understand the use of viral serological studies for the diagnosis of hepatitis A , B & C infections. • To know measures to prevent hepatitis A & B infections. • To know the viral serological tests used to screen blood donors. • Risk of transmission of HBV

  3. Case 1 Mohammed Khan is a 20 year-old male who has recently arrived from India to work as a food handler in a restaurant in Riyadh. Three weeks after his arrival he was seen in A&E Dept. of KKUH because of repeated vomiting, abdominal pain and fever. On examination, his temperature was 38°C, his pulse rate 110/min and BP 120/80mmHg, he was jaundiced and had tenderness in the right upper quadrant of his abdomen.

  4. Case 1 Mohammed Khan is a 20 year-old male who has recently arrived from India to work as a food handler in a restaurant in Riyadh. Three weeks after his arrival he was seen in A&E Dept. of KKUH because of repeated vomiting, abdominal pain and fever. On examination, his temperature was 38°C, his pulse rate 110/min and BP 120/80mmHg, he was jaundiced and had tenderness in the right upper quadrant of his abdomen.

  5. QUESTIONS • What are the possible causes for his presentation? • What investigations would you like to order for him? Explain how these investigations would help you.

  6. Investigation

  7. 3. Based on these findings what is the most likely diagnosis? 4. What further investigations would you like to order?

  8. 5. The serologic results were as follows: 6. Based on the serologic results, what is the diagnosis?

  9. Briefly outline the management of this patient.

  10. Case 2 Mohammed Abdullah is a 34 year old married Saudi male who has donated two units of blood at KKUH for a relative undergoing an operation. Two days later, the Blood Bank called him because of abnormal blood test results and advised him to see his physician. On arrival to the blood bank, the doctor informed him that his blood is not suitable for transfusion because of the presence of infection.

  11. QUESTIONS • What type of infectious agents can be transmitted through blood transfusion? (List 4 infections).

  12. . The next day Mohammed came to see his general practitioner with a letter from the Blood Bank. The letter revealed the result shown below. What is your interpretation? What do you do next?

  13. How would you interpret the results ordered by the GP?

  14. How do you diagnose HCV infection? Serological assay • Screening for (Anti-HCV) by ELISA • Confirmatory test by recombinant immunoblot assay (RIBA) Molecular assay

  15. The General practitioner arrange for him to see hepatologist who examine him and review his results. He further added PCR with genotype for Hepatitis C. What is the significance of these tests & how they can help in the management:

  16. Case 3 A 15-weeks pregnant Saudi woman was seen for the first time at the antenatal clinic at KKUH. As part of the antenatal screening, the doctor arranged for blood screening for viral serology.  

  17. The results were as follows :

  18. How would you interpret these results? • On the lights of these Laboratory results how would you manage the newborn?

  19. 3. Is there a risk of transmission of HBV to the newborn?

  20. 3. Is there a risk of transmission of HBV to the newborn? HBsAg (+) mother 10-20% HBeAg (-) HBsAg (+) mother 90% HBeAg (+)

  21. 4. What further management would you offer to the mother?

  22. Pregnant Hepatitis B carriers should be advised to- Not donate blood, body organs, other tissue. - Not share any personal items that may have blood on them (e.g., toothbrushes ). - Obtain vaccination against hepatitis viruses A as indicated. - Be seen at least annually by their regular medical doctor. - Discuss the risk for transmission with their partner and need for testing.

  23. Today the mother is admitted in labour and you were among the staff involved in the delivery. During a repair of the episiotomy, accidentally you prick your finger with a needle stained by the patient blood?

  24. 1. What should you do? - Report occupational exposures immediately. • The hepatitis B vaccination status and the vaccine-response status (if known) should be reviewed.

  25. 2. What is the risk of infection to you? the risk of developing serologic evidence of HBV inf if the blood (+) HBsAg (+) HBeAg37-62% the risk of developing serologic evidence of HBV inf if the blood (+) HBsAg (-) HBeAg23-37%

  26. * • May be recovering from acute HBV infection. • May be distantly immune and test not sensitive enough to detect very low level of anti-HBs in serum. • May be susceptible with a false positive anti-HBc. • May be undetectable level of HBsAg present in the serum and the person is actually a carrier.

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