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Research Update: Priscu Research Group

Research Update: Priscu Research Group. NGRIP Pink Ice Vostok Ice Drilling Fluid Migration in Ice Particle characterization in ice cores—WAIS DIVIDE (biotic vs abiotic) Lake Vida Project. Basal ”pink” ice from the NorthGRIP core. Photo credits Dorthe Dahl-Jensen.

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Research Update: Priscu Research Group

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  1. Research Update: Priscu Research Group NGRIP Pink Ice Vostok Ice Drilling Fluid Migration in Ice Particle characterization in ice cores—WAIS DIVIDE (biotic vs abiotic) Lake Vida Project

  2. Basal ”pink” ice from the NorthGRIP core Photo credits Dorthe Dahl-Jensen

  3. Subglacial Iron discharge from Blood Falls, Taylor Glacier, MCM Dry Valleys

  4. ethanol ethanol ethanol ethanol glycol NMR Spectra NGRIP ethanol ethanol

  5. NGRIP: “Pink Ice”

  6. Preliminary NGRIP basal ice results DRILLING FLUID CONTAMINATION IN PINK ICE:-100 mM to 20 mM ethanol-Ethylene glycol and peaks in the aromatic/amine and hydrocarbon region -Redrilling of frozen water questionable for scientific assessment PARTICLES IN PINK ICE:-Nearly all material mineral-Grain size fairly homogenous at 1-5mm; subangular -A few larger grains up to ~ 30mm; mainly biotite MINERALS IN PINK ICE :-Quartz, Amphibole, Albite, Hornblende, and K-feldspar with Fe coating and FeOxide coating on biotite.-All primary rock forming minerals, consistent with subglacial till.CELL NUMBERS IN PINK ICE:-1.6±0.38 x 103 cells ml-1 (Sample A3) IN GLACIAL ICE:-some large organic fragments up to 100mm long (not seen in the pink ice)-little mineral material.

  7. Vostok Ice Core Work

  8. -1 -1 cells ml cells ml 0 100 200 300 400 500 0 100 200 300 400 500 600 3510 0 TERM I 500 3530 1000 ACCRETION ICE I 3550 1500 depth (m) 3570 TERM II 2000 2500 3590 depth (meters) 3000 TERM III 3610 ACCRETION ICE II TERM IV 3500 ACCRETION ICE 3630 4000 Propidium Iodide SYBR Gold SYTO 9 (ALIVE) (DEAD) Cell Counts Versus Depth in Vostok 5G depth (meters)

  9. Drilling Fluid (JP8 jet fuel) movement in ice 30 cm

  10. 6.5 cm Tube = 1-2 mm dia

  11. Characterization of particles in ice cores 6 µm calibration beads bacteria abiotic particles Fluorescence/Scatter

  12. Lake Vida Project 2005-06

  13. Characterization of particles in ice cores 100 50 Percentage of cells Cell lysis Nucleic acids Intact Membrane Metabolic activity Light Scatter 0 CTC TOPRO-3 SYTO TIME IN ICE

  14. Vostok core 3622 (~120 above lake water) 16s rDNA clones Anaerobe; uses S or Fe as terminal e- acceptor (>95%) Fe oxidizer? (90%) Obligate methylotroph (97%)

  15. Rapid vertical and lateral movement along grain boundaries

  16. NGRIP: “Pink Ice”

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