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Application of MJ PRRS Vaccine for PRRS Control and Elimination

Application of MJ PRRS Vaccine for PRRS Control and Elimination. Neil DeBuse, DVM. AASV 2010, Omaha, NE. Objectives. Provide brief technical background Grouping Vaccine production process Important areas for monitoring (veterinarian involvement)

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Application of MJ PRRS Vaccine for PRRS Control and Elimination

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  1. Application of MJ PRRS Vaccinefor PRRS Control and Elimination Neil DeBuse, DVM AASV 2010, Omaha, NE

  2. Objectives • Provide brief technical background • Grouping • Vaccine production process • Important areas for monitoring (veterinarian involvement) • Provide overview of vaccine as we have applied it in the field • Quarterly / Sow Unit protocol • Pre-farrow / Sow Unit protocol • Provide a look at our initial results • Sow unit  Results from start to current • Nursery situations with post-exposure vaccination

  3. Previous information • BK Kim, PhD, Microbiologist • Leman Conference 2008 - Making sense of PRRS virus sequences and a new view for PRRS inactivated vaccine – MJ PRRStm: old problem-new approach • www.mjbio.com

  4. Technical Background • Strain Selection • Methodology is patent pending • Grouping; D, S, E x 8 subgroups PRRSV Isolates North American Strains European E-1 Group D Group S E-2 E-3 D-1 2 3 4 5 6 7 8 S-1 2 3 4 5 6 7 8 E-4 E-5 E-6 E-7 E-8

  5. Vaccine Production Process

  6. PRRS Isolate “demographics” SE Minnesota Area 90% of vaccine used is D145, S15 within our veterinary group / 10% with D6

  7. Background • MJ PRRS Vaccine became available in 2007 • A group of 4 veterinary clinics collaborated to use vaccine (common strains) in 4-county area • Dr. Tom Lang • Dr. Mark Engesser • Dr. Mike Strobel • Dr. Neil DeBuse • Collaboration allowed us to use single product, evaluate in various situations and discuss clinical response • Good learning curve • Faster “test”

  8. Sow Unit Protocols • Pre-farrow • Extremely effective colostrum-based protection • Vaccination timing • 8-weeks pre-farrow  aim at protection against late-gestviremia • 8 + 3-weeks pre-farrow  Maximum colostrum; protection into Nursery • Quarterly • Focus for stabilization of sow unit / stop virus circulation • Focus on all sows having equal protection/status • Continue to prevent “re-breaks” with area strains

  9. Case 1: 4 sow units Herd Status at Start • All herds positive, acclimating gilts with one of the two commercial MLV vaccines • Quarterly “blitz” of all sows • Continued PRRS symptoms • Vaccinating 100% of weaned pigs 2x in Nursery (BI ATP)

  10. Case 1: Intervention and Plan Individual Herd Actions • Stop all “ blitz” with live virus • Use conventional mlv for priming gilts • Vaccination whole herd with MJ PRRSTM • Continued Quarterly vaccination 5th quarter • MJ PRRS in gilts • 1st dose - MJ PRRS – 6-8 weeks after priming • 2nd dose 6-8 weeks later • NOTE: All herds had multiple PRRS strain exposure history (D1, D4, D5)

  11. Case 1: Results Herd 1, 2, 3 • PRRS negative weaned pigs within 8 weeks • Weaning consistently negative pigs, throughout 2009 • A single nursery site with PRRS positive results in offsite/downstream (not related to “leaking” virus) • 100% Success Herd 4 • PRRS negative weaned pigs quickly (2 months), but on-site nursery has had sporadic POSITIVES • Partial depopulated nursery rooms • Strict bio-security back from nursery to sow unit • Virus still present in the Nursery @ Oct-2009 • In Nov-09: converted from Quarterly to Pre-Farrowing protocol • Results: Reduced N Mortality & % PCR +

  12. Case 1: Next steps • Monitoring PRRS serology monthly • Herds 1-3 • Continue Quarterly MJ-PRRS use • If 100% weekly PRRS negative pigs through 2010, enter PRRS naive gilts in 2011 • Monitor serologically • If ELISA change or weaned pig change, re-implement or review action plan • Herd 4 • Continue on Pre-Farrow protocol • Eliminate PRRSV at Nursery

  13. Case 2: Active Sow Unit Break Nov/Dec 2008: • Sow unit with 2,500 sows; abortions at 12-18/week for 6 weeks • Vaccine became available and 1x herd administration • Abortions stopped @ 14 days post-vaccine • Negative weaned pigs at 5 weeks Result: • Dramatic results in stopping clinical signs in sows, making negative weaned pigs • Achieved 20 weeks of consistent negative pigs (PCR) • Monitoring smallest weanedpigs -10/week • Stopped monitoring @ 6 mo.

  14. Our Assessment Clinically • Rapid affect on passive immunity • Stop late gestation viremia  reduce in utero infection • Suppression of viremia in herd (population) vs. our previous tactics (lvi) --- We have not performance serum exposure (lvi) in a sow unit since this case. We have adapted to an 8-week prefarrow or 8 + 3 weeks prefarrow depending on nursery flow

  15. Summary – Breeding Herd Application • Successfully taken 18 herds through 2009 • Reduced PRRS virus leakage • Reduced Sporadic Abortion storms • We will have 8-9 of these herds (non-endemic area) go towards elimination in 2010. • We will have 9-10of these herds remain on pre-farrow protocol during 2010. • Developed a better approach to get to PCR negative pigs rapidly • MJ-PRRS vaccine 5-6 weeks vs. • Live virus intervention ~ 12-14 week average (range10-22 wks)

  16. Weaned Pig / Nursery • We have used in limited basis for approx 45,000 pigs • Efforts directed at controlling symptoms and viremia following an outbreak in large Continuous Flow Nursery • Vaccination post-exposure (approx 1 week) • Focus on monitoring room by room • Vaccinate 1 week post exposure (natural infection)

  17. Logic Nursery History Sow unit 1 Sow Unit 2 • PRRS negative system • Purchased weaned pigs • Negative nursery flow • New outbreak • Need for plan to control PRRS • Risk to other finishing sites • Make Nursery negative again Large CF Nursery

  18. Actions Nursery Application Sow unit 1 Sow Unit 2 • Vaccinated outgoing pigs • MJ PRRS post exposure • Reduce finishing impact • 8 groups  N Mort: 16.3% • Vaccinated new groups • 1 week post “clinical” symptoms • Room by room • 9 groups  N Mort: 6.4% Large CF Nursery *

  19. Nursery results PRRS + & vaccine @ exit to F PRRS + & vaccine @ 1 week post clinical

  20. Elimination of PRRS @ Sow Unit level Overall Strategy Without introducing live virus….can we take these steps? Step 1: Ability to produce PRRS negative weaned pigs for > 12 months Step 2: No new clinical signs - No abortions - No PRRS circulation within the sow unit Step 3: Extend to 2 years Step 4: Introduction of PRRS naïve (negative) gilts (a step of herd closure or more knowledge on immunization of naïve gilts may be needed).

  21. Summary • We have a new tool which has been very helpful • Breeding herd • Nursery • Continue to collect and share information within group • Thank you

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