1 / 24

Frozen Vials

Frozen Vials. Normal & abnormal vials can be designated as such w/different colored caps or by manipulating barcodes. Paraffin Sections. A section of tissue is placed in a cassette and fixed in formalin to create a paraffin block. 

ham
Download Presentation

Frozen Vials

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Frozen Vials Normal & abnormal vials can be designated as such w/different colored caps or by manipulating barcodes.

  2. Paraffin Sections • A section of tissue is placed in a cassette and fixed in formalin to create a paraffin block.  • Origin = directly adjacent to frozen section taken from same specimen • “Matched samples” • Stained (H&E) and Unstained Slides • Quality assurance • Diagnosis confirmation • Photography • Archived paraffin retrieval • 10-15 years • Can also create paraffin curls (20μm)

  3. TMAs • Tissue Microarray • Many samples, one marker • Lower cost to researchers than gene microarrays.

  4. OCTs • Tissue placed in small mold • Embedded in Optimum Cutting Temperature (OCT) compound • Once frozen, cube (“chiclets”) is transferred to an air-tight Cryosette ® to prevent dehydration during storage.  • Label Cryosette ® • Pt. representative ID • Sample ID • Generally cut to match paraffin and frozen sections.

  5. Fresh Tissue • Allocate & weigh tissue • Place in correct (pre-marked) vials or self-label tubes • Transport media • Researcher-provided • RPMI w/antibiotic • PBS • Record ischemic time, specimen ID, sample ID, weights, etc. • Package • Notify researcher/send documentation • Pick-up

  6. Blood, Blood Products & Other Fluids • Whole Blood • Serum • Plasma • Synovial Fluid • Aspirates • Smears

  7. Tissue Banking Operations • Sample Data: • Sample ID • Weight of Sample (frozen tissue) • Ischemic time • Case Data: • Case ID • Top line diagnosis • De-identified Pathology report • Pathology Verification • Tumor vs Lesion vs Normal • Percentage of tumor (when present)

  8. Data Tracking w/Excel

  9. Tissue Banking Operations • Inventory management Need to develop both physical and database storage systems • A system for all tissue formats • (Blocks, slides, OCT samples, frozen samples, blood and serum samples) Storing options • Cold storage vs. ambient temperature • Storage boxes • Sample vials • Cryosetts or Mega-cassettes • Blood samples • Labeling • Label printers • Label designed for -180 C • Labeling conventions • Bar coding technology

  10. Freezer • Storage Temperature: (-80) C • Capacity: • 15360 1.2 ml vials • 5400 OCT Cryosetts

  11. Inventory • Tracking Sheets • Bar-coding • Self vs. pre-labeled • Scanner • Excel Spreadsheet • Data transfer • Storage Considerations • Cold Temp. • Ambient Temp.

  12. Tissue Banking Operations • Inventory management • Develop a database for all samples in the inventory • Excel or Access databases (home grown systems) • Dedicated tissue banking software programs are available • caBig (NCI) • Gulfstream • Daedalus • Freezer works “free versions” available • Samples need to be both checked in and checked out when withdrawn from inventory

  13. Inventory List

  14. Tissue Banking Operations • Data Management • Types of pathology and clinical data • Medical records • Pathology reports • Patient interviews • Internal management of pathology reports • What to extract from pathology reports to include with tissue sample • De-identification of pathology reports including names, dates, and case numbers (Software is available for this task). • Clinical Data for Clients • IRB approval of clinical data forms supplied by client. • Extracting information from medical charts vs. patient interviews

  15. Tissue Banking Operations • Data Management • Storage of clinical information and Pathology Data • Commercial systems available • Pathology Information Systems • All information should be based on searchable and retrievable data points (not free text)

  16. Operations • Equipment needs • Revco (-80 degree Celsius freezer) • Scale (to at least 100 mg) • Label printer with labels that can withstand -180 • Source / storage of liquid nitrogen (Cryoshipper) • Computer / Printer

  17. Operations • Personnel requirements / Staffing • What is the scope of the Tissue Banking Program • One man show vs. a full scale operation • What support can come for other departments • Is the tissue bank supporting both internal research, external research or both • Capacity

  18. Operations • Financial considerations • Funding possibilities • Hospital commitment to the effort • Pharmaceutical collaborations • Tissue brokers collaborations • Internal research grants / Department support • Academic collaborations • National grants

  19. “Tasks” • Determine what case types to bank • Review surgical schedule to identify desirable cases • Meeting and consenting patients • Communication with the OR to identify targeted case and to arrange for timely specimen transport • Handling the tissue/blood (Dividing and processing for various sample formats) • Labeling samples and placing them into an inventory • Maintaining the inventory (Update deposits and withdraws) • Processing of histology blocks • Pathology verification (tumor percent, ischemic time, sample weight) • Develop / purchase a searchable database that can hold case information • Data collection (Pathology report and clinical data retrieval) • De-identification of all patient demographics • Shipping or transporting samples to the researcher • Office responsibilities • Billing management for both external accounts. • Equipment and supply needs

  20. Thanks!

  21. Contact Information • Mike Jones, MD Principal Investigator jonesm@mmc.org 207-662-2959 • Paul Auclair, DMD • auclap@mmc.org 207-662-4025 • Rocky Ackroyd, Path Ass’t • ackror@mmc.org 207-662-6825 Web Site: www.mmctissuebank.org

More Related