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Nasonia vitripenni. You are the PI ( P rimary I nvestigator). Jewel Wasp. Tell me about it…. Life Cycles. Haplo/Diplo Genetics. Wasp Development Timelines 18 o , 28 o C. Complementation. Process by which an allele of each of two mutant genes makes up for the defect in the other gene.
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Nasonia vitripenni You are the PI (Primary Investigator)
Jewel Wasp Tell me about it…
Complementation Process by which an allele of each of two mutant genes makes up for the defect in the other gene.
You Find Three Mutants With a Similar Phenotype Are they the Same Genotype?
Possible Physiology Enzyme A Enzyme A Enzyme A Enzyme A Enzyme B Enzyme B Enzyme B Enzyme B transport Enzyme C Enzyme C Enzyme C Enzyme C
Complement a | | B a | | B A | | b A | | b x a | | B A | | b Two genes Both Recessive Think about dominant mutations!
Fail to Complement A | | b A | | b A | | b A | | b x A | | b A | | b 1 gene
Three scarlet eyed mutants, how many loci?stDR,stC,st318 stDR Connect same gene with a line. For example. Fail to complement stC st318
Scoring Red Eyes Here
Wasp Business Link
To DoFriday • Plan experiment! • define symbols, diagram crosses, determine expectations, etc. • Each student: complementation. • Per bench, or per pair (will tell next week), • recessive, dominance, • controls (stock maintenance). To Do Friday -Wednesday? …up to you!
Plan Your Crosses Think about the wasp’s schedule, Think about your schedule, Plan accordingly!
April 14 - 23 • Finish C. elegans experiment, • Tinker with wasps, • Exam Review (April 20th) • Exam I (April 23rd), • Basic Mendel! • Basic Mendel! • Basic Mendel! • Basic Mendel! • Basic Mendel! • C. elegans! • Bioinfomatics! • Nasonia! No Lecture April 13th