Chapter 8 Leukocyte Differentiantion Antigens and Cell Adhesion Molecules

1. Chapter 8Leukocyte Differentiantion Antigens and Cell Adhesion Molecules

2. Contents • PartⅠ Membrane molecules of immune cell • PartⅡ Definition of leukocyte differentiation antigen and CD • PartⅢ Definition, classification and functions of adhesion molecules • PartⅣ Clinical application of CD and adhesion molecules

3. PartⅠ Membrane molecules of immune cell • Receptors: TCR, BCR, CR, CKR, FcR • ClassⅠand classⅡ MHC molecules • CD molecules: CD1~339 • Cell Adhesion Molecules

4. PartⅡ Definition of leukocyte differentiation antigen and CD • Leukocyte differentiation antigen: Cell surface molecules expressed (or disappeared) during different developmental and differential phases, activation or inactivation process of blood cells.

6. PartⅡ Definition of leukocyte differentiation antigen and CD • Leukocyte differentiation antigen • CD: cluster of differentiation. The same differentiation antigen recognized by different monoclonal antibody from different lab are called CD.

7. CDs which take part in T cell recognition, adhesion and activation

8. CDs which take part in B cell recognition, adhesion and activation

9. PartⅢ Definition, classification and functions of adhesion molecules

10. Ⅰ. Definition • Cell adhesion molecules (CAMs) are cell surface proteins involved in the interaction of cell-cell or cell-extracellular matrix. • CAMs take effect by the binding of receptor and ligand.

11. Ⅱ. Classification • Integrin family • Selectin family • Immunoglobulin (Ig) superfamily • Cadherin family • Mucin-like family • Other adhesion molecules

12. 1. Integrin family • Integrins consist of α and β chains. • According to β subunits, Integrins are divided into eight groups: β1- β8 • VLA-4(Very Late Antigen-4)------VCAM-1 LFA-1(Lymphocyte Function-associated Antigen-1)------ICAM-1,2,3 • MAdCAM-1 (Mucosal Addressin Cell Adhesion Molecule-1) • TSP-1 ((Thrombospondin一1):凝血酶敏感蛋白一1

17. Integrin and platelet aggregation (Karp, 2001)

18. Integrinspromote immune response

19. 2. Selectin family • Selectins consist of one peptide chain. • The three family members include: E-selectin, L-selectin, and P-selectin.

24. 3. Ig superfamily(IgSF) • The structure of these adhesion molecules resemble that of Ig. • CD4, CD8, CD2(LFA-2), CD58(LFA-3), VCAM-1, ICAM-1,2,3

30. 4. Cadherin family • E-cadherin------ Epithelia cell • N-cadherin------ Nerve cell • P-cadherin-------Placenta

32. 5. Mucin-like familyCD34, GlyCAM-1(glycosylation dependent cell adhesion molecule-1) PSGL-1(P-selectin glycoprotein ligand-1) 6. Other adhesion moleculesCD44

33. Ⅲ. Functions • Participate in development and differentiation of immune cells • CD2----LFA-3 • LFA-1----ICAM-1 -------Participate in development and maturation of thymocytes.

34. 2.Participate in immune response and regulation

36. 3. Participate in the adhesion of leukocyte and vascular endothelial cell during inflammation.

37. 4. Participate in lymphocyte homing • Lymphoid stem cell migrate to central lymphoid organs. • Mature lymphocyte migrate to peripheral lymphoid organs • Recirculation of lymphocytes • Lymphocyte migrate to the sites of inflammation

39. 5. Participate in regulation of apoptosis • Integrins inhibit cell apoptosis

40. What should you know by the end of this lecture? • Definition of leukocyte differentiation Antigen,CD and adhesion molecules • Classification and function of adhesion molecules