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∆ laeA veA1. OE:: rsmA ∆laeAveA1. OE:: rsmA veA1. WT veA1. ST. Light. Dark. Supplementary Figure 1.

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  1. ∆laeA veA1 OE:: rsmA ∆laeAveA1 OE:: rsmA veA1 WT veA1 ST Light Dark Supplementary Figure 1. Thin layer chromatography of chloroform extracts from WT, OE::rsmAveA1, ∆laeAveA1 and OE::rsmA∆laeAveA1 (RDIT2.3, RMS11.1, RJW46.4 and RMS53.1 respectively) at dark and light. OE::rsmAveA1 has high impact on ST and secondary metabolite production with remediation of ST production of ∆laeA mutant either at dark or light. (ST: sterigmatocystin standard).

  2. ST ∆laeA veA OE:: rsmA ∆laeAveA OE:: rsmA veA WT veA Light Dark Supplementary Figure 2. Thin layer chromatography of chloroform extracts from wild type (WT), OE::rsmA, ∆laeAveA and OE::rsmA∆laeAveA (RDIT9.32, RMS4.25, RJW41.A and RMS41.1) respectively. OE::rsmAveA has greatly enhanced ST and secondary metabolite production with remediation of ST production of ∆laeA mutant at dark.(ST: sterigmatocystin standard).

  3. Light Dark OE::rsmA ∆veA OE::rsmA ∆veA ∆veA ∆veA ST ST Supplementary Figure 3. Thin layer chromatography of chloroform extracts of ST production by ∆veA and OE::rsmA∆veA (RJW112.2 and RMS4.1696 respectively) from solid GMM. OE::rsmA∆veA has greatly enhanced ST and secondary metabolite production with remediation of ST production of ∆veA mutant at both dark and light.(ST: sterigmatocystin standard).

  4. 2500000000 a 2 Day 7 - Champes 2000000000 b 1500000000 a Sexual spores/mm a 1000000000 b b c c 500000000 0 WT WT DlaeA, veA DlaeA, veA OE::rsmA, veA OE::rsmA, veA OE::rsmA, DlaeA, veA OE::rsmA, DlaeA, veA Light Dark 5.00E+07 a Day 7 - GMM 4.50E+07 4.00E+07 a 2 3.50E+07 3.00E+07 2.50E+07 Sexual spores/mm 2.00E+07 1.50E+07 b 1.00E+07 a ab b 5.00E+06 b c 0.00E+00 WT DlaeA, OE::rsmA, OE::rsmA, WT DlaeA, OE::rsmA, OE::rsmA, veA veA DlaeA, veA veA DlaeA, veA veA Supplementary Figure 4. Overexpression of rsmA does not remediate sexual defects in DlaeA. Sexual development on Champes or GMM media. The DlaeA mutant produces the same number of ascopores in dark and light regimes when grown on Champes medium. WT (RDIT9.32), DlaeA,veA (RJW41.A), OE::rsmA, veA (RMS4.25), OE::rsmA,DlaeA,veA (RMS41.1). Media was inoculated with 1X106 spores and incubated for 7 days in the light and dark. Error bars designate standard deviations. Letters (a-c) indicate differences between strains within a light regime (e.g. light on Champes medium) with statistical significance (P< 0.05) according to the Tukey-Kramer multiple comparison test.

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