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Biotechnology. Mammalian Cloning. Somatic cell nuclear transfer. Stem Cells. AATTCGGCCATATACG GCCGGTATATGCTTAA. Desired Gene. Restriction Sites for EcoRI. Restriction Enzymes. Bacterial defense against viral DNA Cut DNA at specific sequences.
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Mammalian Cloning Somatic cell nuclear transfer
AATTCGGCCATATACG GCCGGTATATGCTTAA Desired Gene Restriction Sites for EcoRI Restriction Enzymes • Bacterial defense against viral DNA • Cut DNA at specific sequences CCTTTG AATTCCCAGAATC GGAAACTTAA GGGTCTTAG
Electrophoresis • Separation of molecules based on size • Negatively charged DNA molecules are pulled through a gel by an electrical field • Smaller molecules travel faster and farther
DNA Fingerprinting Variations in DNA Variations in restriction sites Variations in fragment sizes Variations in electrophoresis bands
Poop Walter Jordan
GeneCloning Gene Splicing Transformation Binary Fission