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Review

Review. Lab Exercises 8-11. Exercise 8. What is the purpose of this exercise? What is a capsule? What is the capsule made of? What is the importance of the capsule? Describe the staining procedure for staining the capsule What bacteria we use for the capsule stain procedure?.

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Review

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  1. Review Lab Exercises 8-11

  2. Exercise 8 • What is the purpose of this exercise? • What is a capsule? • What is the capsule made of? • What is the importance of the capsule? • Describe the staining procedure for staining the capsule • What bacteria we use for the capsule stain procedure?

  3. Exercise 8 cont. • What is a flagella? • What is taxis? • Why do we stain the flagella? • What bacteria we observed the flagella? • What is an endospore? • Why do bacteria produce spores? • Describe the composition of the endospore

  4. Exercise 8 cont. • When do endospores dissolve? • Describe the procedure to stain endospores • What bacteria we use for the endospore stain procedure? • What is mycolic acid? • What antibiotics are used against TB? • Describe the procedure for acid fast stain • What bacterias we use for this staining procedure?

  5. Exercise 9 • What is the purpose of the exercise? • Explain the major steps involved in obtaining pure cultures from a mixed population • Explain the difference between selective and differential media • Describe the appearance of the following bacteria in EMB • E. coli, Enterobacter, Klebsiella, Proteus, Salmonella, Shigella • Explain the color changes on EMB

  6. Exercise 9 cont. • Describe the growth of the following bacterias on TSA • M. luteus, S. epiderdimis, E. coli, E. aerogenes • Describe the growth of the following bacterias on EMB • M. luteus, S. epiderdimis, E. coli, E. aerogenes • Describe the growth of the following bacterias on CNA • M. luteus, S. epiderdimis, E. coli, E. aerogenes

  7. Exercise 10 • What is the purpose of this exercise? • Explain the composition of the blood agar? • Differentiate between beta, alpha, and gamma hemolysis on blood agar • Describe the composition and use of Mannitol Salt Agar (MSA) • Explain the catalase test

  8. Exercise 10 cont. • Explain the coagulase test • Explain the bacitracin or Taxo A disc test • Describe the composition and use of the Bile Esculin Agar • Explain the 6.5% NaCl testing • Explain the Optochin or Taxo P disc test

  9. Exercise 10 cont. • Describe the growth on blood Agar of: • S. aureus, S. epidermidis, E. faecalis, S. bovis • Describe the growth on MSA of: • St. aureus, S. epidermidis, E. coli • Describe the coagulase test on S. aureus and S. epidermidis • Describe the growth of E. faecalis and S. bovis on Bile Esculin Agar

  10. Exercise 10 cont. • Describe the growth of S. bovis and E. faecalis on 6.5% NaCl • Describe the growth of S. pyogenes with Taxo A • Describe the growth of S. pneumoniae with Taxo P

  11. Exercise 11 • What is the purpose of the exercise? • How do you differentiate Staphylococcus and Streptococcus. • How do you know if you have S. pyogenes in your throat?

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