380 likes | 640 Views
Identifying and Classifying Bacteria. Ch. 23 . What is a prokaryote?. Cells that lack a true nucleus . Cells that lack membrane-bound organelles . Most surrounded by a cell wall. Many secrete a protective slime capsule . How big is a prokaryotic cell?. 1- 5 micrometers
E N D
What is a prokaryote? • Cells that lack a true nucleus. • Cells that lack membrane-bound organelles. • Most surrounded by a cell wall. • Many secrete a protective slime capsule.
How big is a prokaryotic cell? • 1- 5 micrometers • (1 millionth of a meter)
Classification • Archaebacteria vs Eubacteria
Archaebacteria • Surrounded by a cell wall lacking peptidoglycan. • Live in extreme conditions
Examples of Archae • Methanogens: convert H2 and CO2 into methane gas for energy • Halophiles: love salt • Thermoacidophiles: love heat and acid
Eubacteria: • Surrounded by a cell wall made of peptidoglycan. • Example: rhizobium- nitrogen–fixing bacteria
Identifying Prokaryotes 1. Shape: • Coccus – spherical • Bacillus – rod-shaped • Spirillum – spiral • Vibrio – comma shape
Bacillus Coccus Vibrio Spirillum
Cocci and Bacilli may group together Prefixes for arrangements: Diplo – two Ex. Diplococcus
Strepto – chain Streptococcus Streptobacillus
Staphylo - clustered Staphylococcus
2. Motility: ability to move • Flagella: whip-like structure used to propel bacteria • Slime: glide along slime secretion • Spiral motion: cork-screw motion (spirillum)
3. Metabolism: Oxygen or NOT • Obligate Aerobes: Need oxygen • Obligate Anaerobes: cannot live in oxygen • Facultative anaerobes: can live with or without oxygen
3.Metabolism: Obtaining Energy Autotroph vs. Heterotroph • Photoautotroph- uses light energy for photosynthesis • Chemoautotroph- uses energy from inorganic chemicals for chemosynthesis
Photoheterotrophs- uses photosynthesis and eats organic compounds • Heterotroph – consumes organic compounds
review • What role do chemosynthetic bacteria have in the ecosystem? • http://www.youtube.com/watch?v=4lmwbBzClAc&NR=1
Asexual: Binary fission • Circular DNA replicates • Cell membrane and cell wall divide • Identical daughter cells separate
How fast does this happen? • http://www.youtube.com/watch?v=gEwzDydciWc&feature=related • approximately every 20 minutes
Genetic recombination Sharing or exchanging of genetic material 3 types: • Conjugation • Transformation • Transduction
1. Conjugation • A temporary bridge forms between two cells. • A plasmid, a separate section of DNA, is transferred from one cell to the other. • Often the plasmid contains useful genes, like antibiotic resistance.
2. Transformation • Bacteria takes in DNA from the environment. • Dead bacteria may break apart and release DNA. • Other bacteria can pick up this DNA and become genetically different.
3. Transduction • Viruses can pick up small amounts of DNA from a host bacteria. • When it infects another bacteria, the new DNA is transferred into that cell.
Disease • Pathology is the study of disease. • Pathogens: organisms that cause disease.
Gram-staining Test • Gram-positive: bacteria contain lots of peptidoglycan in cell walls. Stains purple. • Gram- negative: bacteria contain little peptidoglycan in cell walls. Stains red.
Exotoxins • Poisonous proteins that are released by some gram-positive bacteria.
For example • Clostridium tetani secretes an exotoxin that causes tetanus. • Tetanus causes stiffness in muscles. • Clostridium botulinumproduces a very powerful exotoxin that causes the fatal disease, botulism. • 1 g of botulism toxin can kill 1 million people.
Endotoxins • Lipids and carbohydrates in the cell membranes of some Gram-negative bacteria, that are poisonous. • They are released when the bacteria die.
For example: • Most species of Salmonella, are endotoxin producing bacteria. • Salmonellatyphi, causes typhoid fever.
Antibiotics • Antibiotics interfere with the bacteria’s cellular activities.
For example: • Penicillin: blocks the building of the cell wall. “Accidently” discovered by Alexander Fleming in 1920s • Tetracyline: blocks protein synthesis.
Where do antibiotics come from? • Antibiotics are naturally made by some fungi and bacteria. • Some are made synthetically in labs. • But most used in treatment still come from bacteria.
Antibiotic-Resistance • Bacteria can quickly adapt to their environment because of their rapid rate of reproduction.
Antibiotic-Resistance • If one bacterium mutates and becomes resistant, • then in the presence of antibiotics, bacteria not resistant die • Those with the resistance take over the population.
Which diseases are preventable by better sanitation practice? • Cholera • Salmonella • Tetanus • Staph
What are other ways that antibiotic resistance is passed? • Genetic recombination