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Identifying and Classifying Bacteria

Identifying and Classifying Bacteria. Ch. 23 . What is a prokaryote?. Cells that lack a true nucleus . Cells that lack membrane-bound organelles . Most surrounded by a cell wall. Many secrete a protective slime capsule . How big is a prokaryotic cell?. 1- 5 micrometers

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Identifying and Classifying Bacteria

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  1. Identifying and Classifying Bacteria Ch. 23

  2. What is a prokaryote? • Cells that lack a true nucleus. • Cells that lack membrane-bound organelles. • Most surrounded by a cell wall. • Many secrete a protective slime capsule.

  3. How big is a prokaryotic cell? • 1- 5 micrometers • (1 millionth of a meter)

  4. Classification • Archaebacteria vs Eubacteria

  5. Archaebacteria • Surrounded by a cell wall lacking peptidoglycan. • Live in extreme conditions

  6. Examples of Archae • Methanogens: convert H2 and CO2 into methane gas for energy • Halophiles: love salt • Thermoacidophiles: love heat and acid

  7. Eubacteria: • Surrounded by a cell wall made of peptidoglycan. • Example: rhizobium- nitrogen–fixing bacteria

  8. Identifying Prokaryotes 1. Shape: • Coccus – spherical • Bacillus – rod-shaped • Spirillum – spiral • Vibrio – comma shape

  9. Bacillus Coccus Vibrio Spirillum

  10. Cocci and Bacilli may group together Prefixes for arrangements: Diplo – two Ex. Diplococcus

  11. Strepto – chain Streptococcus Streptobacillus

  12. Staphylo - clustered Staphylococcus

  13. 2. Motility: ability to move • Flagella: whip-like structure used to propel bacteria • Slime: glide along slime secretion • Spiral motion: cork-screw motion (spirillum)

  14. 3. Metabolism: Oxygen or NOT • Obligate Aerobes: Need oxygen • Obligate Anaerobes: cannot live in oxygen • Facultative anaerobes: can live with or without oxygen

  15. 3.Metabolism: Obtaining Energy Autotroph vs. Heterotroph • Photoautotroph- uses light energy for photosynthesis • Chemoautotroph- uses energy from inorganic chemicals for chemosynthesis

  16. Photoheterotrophs- uses photosynthesis and eats organic compounds • Heterotroph – consumes organic compounds

  17. review • What role do chemosynthetic bacteria have in the ecosystem? • http://www.youtube.com/watch?v=4lmwbBzClAc&NR=1

  18. Bacterial Reproduction

  19. Asexual: Binary fission • Circular DNA replicates • Cell membrane and cell wall divide • Identical daughter cells separate

  20. How fast does this happen? • http://www.youtube.com/watch?v=gEwzDydciWc&feature=related • approximately every 20 minutes

  21. Genetic recombination Sharing or exchanging of genetic material 3 types: • Conjugation • Transformation • Transduction

  22. 1. Conjugation • A temporary bridge forms between two cells. • A plasmid, a separate section of DNA, is transferred from one cell to the other. • Often the plasmid contains useful genes, like antibiotic resistance.

  23. 2. Transformation • Bacteria takes in DNA from the environment. • Dead bacteria may break apart and release DNA. • Other bacteria can pick up this DNA and become genetically different.

  24. 3. Transduction • Viruses can pick up small amounts of DNA from a host bacteria. • When it infects another bacteria, the new DNA is transferred into that cell.

  25. Disease • Pathology is the study of disease. • Pathogens: organisms that cause disease.

  26. Gram-staining Test • Gram-positive: bacteria contain lots of peptidoglycan in cell walls. Stains purple. • Gram- negative: bacteria contain little peptidoglycan in cell walls. Stains red.

  27. Exotoxins • Poisonous proteins that are released by some gram-positive bacteria.

  28. For example • Clostridium tetani secretes an exotoxin that causes tetanus. • Tetanus causes stiffness in muscles. • Clostridium botulinumproduces a very powerful exotoxin that causes the fatal disease, botulism. • 1 g of botulism toxin can kill 1 million people.

  29. Endotoxins • Lipids and carbohydrates in the cell membranes of some Gram-negative bacteria, that are poisonous. • They are released when the bacteria die.

  30. For example: • Most species of Salmonella, are endotoxin producing bacteria. • Salmonellatyphi, causes typhoid fever.

  31. Antibiotics • Antibiotics interfere with the bacteria’s cellular activities.

  32. For example: • Penicillin: blocks the building of the cell wall. “Accidently” discovered by Alexander Fleming in 1920s • Tetracyline: blocks protein synthesis.

  33. Where do antibiotics come from? • Antibiotics are naturally made by some fungi and bacteria. • Some are made synthetically in labs. • But most used in treatment still come from bacteria.

  34. Antibiotic-Resistance • Bacteria can quickly adapt to their environment because of their rapid rate of reproduction.

  35. Antibiotic-Resistance • If one bacterium mutates and becomes resistant, • then in the presence of antibiotics, bacteria not resistant die • Those with the resistance take over the population.

  36. Which diseases are preventable by better sanitation practice? • Cholera • Salmonella • Tetanus • Staph

  37. What are other ways that antibiotic resistance is passed? • Genetic recombination

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