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Oxidation and Reduction of Hemoglobin

Oxidation and Reduction of Hemoglobin. CHEM-342 Introduction to Biochemistry. Reversible “Reduction” of Oxyhemoglobin. Add a small amount of sodium dithionite, Na 2 S 2 O 4. Stir in the presence of air. Spectra for Oxy- and Deoxy -Hemoglobin. OxyHb. DeoxyHb. OxyHb.

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Oxidation and Reduction of Hemoglobin

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  1. Oxidation and Reduction of Hemoglobin CHEM-342 Introduction to Biochemistry

  2. Reversible “Reduction” of Oxyhemoglobin Add a small amount of sodium dithionite, Na2S2O4 Stir in the presence of air

  3. Spectra for Oxy- and Deoxy-Hemoglobin OxyHb DeoxyHb OxyHb Wavelength (nm) DeoxyHb

  4. Oxidation of Oxyhemoglobinto Methemoglobin Add a small amount of Potassium ferricyanide, K3[Fe(CN)6] OxyHb MetHb

  5. Spectra for Carbonmonoxy- and Met-Hemoglobin CO-Hb Met-Hb Wavelength (nm) Met-Hb

  6. Redox Reactions of Hemoglobin -O2 -O2 +O2 -e- OxyHb Oxidizing agents [-e-] Reducing agents [+e-] DeoxyHb MetHb

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