1 / 13

MLAB 2401: Clinical Chemistry Keri Brophy -Martinez

MLAB 2401: Clinical Chemistry Keri Brophy -Martinez. Methods of Measurement: Mineral and Bone Metabolism. Determination of Total Calcium. Colorimetric O-cresolphthalein Calcium in alkaline solution reacts with o-cresolphthalein complexone to form a purple complex

herman
Download Presentation

MLAB 2401: Clinical Chemistry Keri Brophy -Martinez

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. MLAB 2401: Clinical ChemistryKeri Brophy-Martinez Methods of Measurement: Mineral and Bone Metabolism

  2. Determination of Total Calcium • Colorimetric • O-cresolphthalein • Calcium in alkaline solution reacts with o-cresolphthalein complexone to form a purple complex • Proportional to the calcium concentration • Uses 8-quinolinol or 8-hydroxyquioline to remove Mg ions • Arsenzo III dye • Atomic Absorption Spectrophotometry • Reference method

  3. Specimen Requirements:Calcium( total) • Serum • Lithium heparin plasma • Avoid the use of anticoagulants with EDTA or oxalate

  4. Determination of Free Calcium • Ion selective electrodes used • pH dependent • Selectively and reversibly bind calcium ions • As calcium binds to the membrane a potential develops which is proportional to the concentration

  5. Specimen Requirements:Calcium (Ionized) • Samples must be collected anaerobically • Heparinized whole blood preferred • Plasma • Serum

  6. Reference Ranges • Calcium, total • 8.60-10.0 mg/dL • Ionized Calcium • 4.60-5.08 mg/dL

  7. Determination of Phosphorus • Formation of ammonium phosphomolybdate complex • Complex measured by UV absorption at 340 nm or reduced to molybdenum blue and read between 600-700 nm

  8. Specimen Requirements: Phosphorus • Serum • Lithium heparin plasma • Avoid hemolysis and certain anticoagulants • Diural variation a factor

  9. Reference Range: Phosphorus • 2.5-4.5 mg/dL

  10. Determination of Magnesium • Colorimetric • Calmagite • Mg2+ binds with calmagite to for a reddish-violet complex, read at 532 nm • Formazen dye • Mg2+ binds with the dye to form a colored product read at 660 nm • Methylthymol blue • Mg2+ binds with the chromogen to form a colored complex • Atomic Absorption Spectrophotometry • Reference method

  11. Specimen Requirements: Magnesium • Serum • Heparinized plasma • Avoid hemolysis • Remove serum from cells ASAP

  12. Reference Range: Magnesium • 1.6-2.6 mg/dL

  13. References • Bishop, M., Fody, E., & Schoeff, l. (2010). Clinical Chemistry: Techniques, principles, Correlations. Baltimore: Wolters Kluwer Lippincott Williams & Wilkins. • Sunheimer, R., & Graves, L. (2010). Clinical Laboratory Chemistry. Upper Saddle River: Pearson .

More Related