1 / 2

1 2 3 4

Exercise 14. Plasmid Isolation and Restriction Enzyme digest. 1 2 3 4. 1 : Uncut plasmid DNA 2 : DraI digested ( Master mix D) 3 : EcoRI digested ( Master mix E) 4 : 1Kb DNA ladder. Dr. H. Park. Amp R. Cml R. pCSLA1. EcoRI.

hesper
Download Presentation

1 2 3 4

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Exercise 14. Plasmid Isolation and Restriction Enzyme digest 1 2 3 4 1 : Uncut plasmid DNA 2 : DraI digested ( Master mix D) 3 : EcoRI digested ( Master mix E) 4 : 1Kb DNA ladder Dr. H. Park

  2. AmpR CmlR pCSLA1 EcoRI ~800 bp insert EcoRI There are multiple DraI sites on the plasmid Plasmid itself is about 6.4 kb in length (without any insert) With insert here of 0.8 kb, it should be around 7.2 kb.

More Related