1 / 4

Estimation of carbohydrate by Nelsons’s Method II

Molybdenum (Blue Complex). + Cu² +. + Cu +. + Cu +. + Cu² +. Gluconic acid. PMA. Estimation of carbohydrate by Nelsons’s Method II. Cupric ions are reduced by sugars to cuprous ions, which quantitatively reduce phosphomolybdic acid to a blue complex. Materials.

hesper
Download Presentation

Estimation of carbohydrate by Nelsons’s Method II

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Molybdenum (Blue Complex) + Cu²+ + Cu+ + Cu+ + Cu²+ Gluconic acid PMA Estimation of carbohydrate by Nelsons’s Method II • Cupric ions are reduced by sugars to cuprous ions, which quantitatively reduce phosphomolybdic acid to a blue complex.

  2. Materials 1. Alkaline copper reagent. 2. Phosphomolybdic acid reagent. 3. Standard glucose solution containing 1mg/ml. 4. Glucose solution of unknown strength( 30-70 μg/ml). 5. Colorimeter or spectrophotometer.

  3. 60 5 55 10 50 45 15 40 20 35 25 30 Tube # Blank 1 2 3 4 5 6 unk. 2 min. 8 min. Glucose (ml) 0 0.25 0.5 0.75 1 1.5 2 2 D. W. (ml) 2 1.75 1.51.25 1 0.5 0 0 Alkaline Cu 2 2 2 2 2 22 2 Reagent (ml) PMA 2 2 2 2 2 2 2 2 Reagent (ml) COOL Shake Well D. W. (ml) 4 4 44 4 4 4 4 Read the Optical Density at 630 nm

  4. Results Samar A. Damiati

More Related