Name:_______________

1. Quiz 8: Biol 203 Fall 2013 The Arristaless-like (Arl) gene below carries a homeodomain DNA binding domain (HD). Furthermore, it has a proven activator domain (A) and repressor domain (R). Possible WT mRNAs with A-HD, which have activator-like properties as a protein, promote cell growth Possible WT mRNAs with R-HD, which have repressor-like properties as a protein, inhibit cell growth. mRNAs with HD alone never occur. WT mRNAs with A-R-HD code for non-functional proteins. Name:_______________ Lab:_______________ 20pts Arl HD R A E1 E2 E3 1 2 means enhancer binding site Express TxFactor E1 RBP means RNA binding protein Express TxFactors E1, E2 RBP binding sites (where RBP would bind in the transcribed RNA) Express TxFactors E1, E2, E3 Q1(15pts). If RBP typeA suppresses 3’ acceptor site usage in the unspliced RNA at position 1, then mark cells below where R-HD mRNAs will be made. If RBP typeB suppresses 3’ acceptor site usage in the unspliced RNA at position 2, then mark cells below where A-HD mRNAs will be made. E1 and E2 are both needed to drive RBP typeA expression E1, E2, and E3 (all3) needed to drive RBP typeB expression E1, E2, and E3 (all3) needed to drive Arl expression 30 cells in WT Antennal Tissue 5pts 5pts 5pts A null allele within a RBP gene will give the same phenotype within the 30 cells as a null allele for Arl. Which one? RBP-B Q2(5pts- 1pt each). Using a flow chart and a few words: please describe how first strand cDNA is derived from an mRNA? mRNA oligo dT primer plus Reverse transcriptase 5’---------------------------3’ 5’---------------------------3’ What does the first 5’ nucleotide of this first strand cDNA correspond to in the mRNA?_pA tail, A______________ What does the last 3’ nucleotide of this first strand cDNA correspond to in the mRNA? _TSS, gCAP, 1st nucleotide of exon 1,5’UTR____ What does the first 5’ nucleotide of this first strand cDNA correspond to in the gDNA? __nothing______ What does the last 3’ nucleotide of this first strand cDNA correspond to in the gDNA? _nothing, TSS, 1st nucleotide of exon1,5’UTR___