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CATALASE ENZYME

CATALASE ENZYME. A Ainun Muflika (20512043) Cut Yuliana (20512058) Biochemistry 2012. Content. Struktur dan Reaksi. Penamaan enzim Enzim katalase (EC.1.11.1.6) merupakan enzim kelas oksidoreduktase. Struktur Enzim katalase. Reaksi enzim katalase. ISOLASI.

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CATALASE ENZYME

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  1. CATALASE ENZYME A AinunMuflika (20512043) Cut Yuliana (20512058) Biochemistry 2012

  2. Content

  3. StrukturdanReaksi Penamaanenzim Enzimkatalase (EC.1.11.1.6) merupakanenzimkelasoksidoreduktase StrukturEnzimkatalase

  4. Reaksienzimkatalase

  5. ISOLASI Terdapatdalamhatimamalia, peroksisomtumbuhan, semuabakteriaerob, danjamur SalahsatujenisjamuradalahNeurosporacrassa. CAT 1 merupakanenzim yang diisolasidarijenisjamurtersebutdenganmenggunakanteknikkromatografi. Dalamsebuahzymogramterdapat 5 aktivitas pita terdiridari CAT 1a enzim yang tidakteroksidasi, CAT 1e enzim yang teroksidasipenuhsedangkan CAT 1b – CAT 1d enzimdalambentukintermediet. Tingkat keasamanpada CAT 1a lebihrendah (PI 5,45) daripada CAT 1e (PI 5,25).

  6. KINETIKA ENZIM KATALASE Konstantakinetikpadaaktivitasenzim CAT 1a dan CAT 1e dilakukanpadasuhu 22oC denganmenentukankecepatanteramatipadapelepasan gas O2denganmenggunakanmetodeElektroda Clark.

  7. Aktivitasenzim CAT 1a dan CAT 1e diujipangaruhnyaterhadap pH yaitupadarentang pH 3-8

  8. Aktivitasenzim CAT 1a dan CAT 1e diujidenganmenggunakan inhibitor dimanaInhibibitor yang digunakanyaitu KCN dengankonsentrasi yang berbeda-bedadenganrentang 0,4-1mM

  9. APLIKASI

  10. MODIFIKASI

  11. DAFTAR PUSTAKA Diaz, A, et.al. 2005. Functionaland structural analysis of catalase oxidized by singlet oxygen. Journal biochemistry vol 87 : 205–214. Meksiko. Kangralkal, et.al. 2010. Oxidative stress and diabeters: a review. International Journal of PharmaticalApplications. Vol 1,:38-45. India Chang Dong et.al. 2012. Association of Catalase Genotype with Oxidative Stress in Predication of Colorectal Cancer : Modification by Epidemiological Factors. Biomed Eviron Sci. 25(2):156-162. China Maksimenko, et.al. 1997. Modification of Catalase by Chodroitin Sulfate. CRS. Rusia Worthinghon Biochemical Corporation . 2009. “Catalase”. Worthinghon Enzyme Manual. Mexico Loewen,P. 1996. Probing the Structureof Catalase HPII of Eschericha coli-a review. Gene Elsevier : 39-44. Canada

  12. TERIMA KASIH

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