Figure 1. Pulse field gel electrophoresis of CA-MSSA isolates

Oct 29, 2014

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Figure 1. Pulse field gel electrophoresis of CA-MSSA isolates.

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Figure 1. Pulse field gel electrophoresis of CA-MSSA isolates

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Figure 1. Pulse field gel electrophoresis of CA-MSSA isolates

GEL ELECTROPHORESIS:

GEL ELECTROPHORESIS:. The method for separation and purification of nucleic acids and proteins. Electrophoresis of Onion DNA. The purpose of this lab is to first isolate onion DNA by lysis and then separate and isolate the individual nucleic acids of the onion DNA by gel electrophoresis. .

796 views • 26 slides

Gel Electrophoresis

699 views • 19 slides

Gel Electrophoresis

Contents. Basic PrinciplesThe Electric FieldThe Electrophoretic Mobility ValueThe GelsImproving ResultsMethods of SeparationManipulating the GelsNew Techniques. Principles of Electrophoresis. Separation based upon several factorsMolecular chargeMolecular massMolecular shapeMolecules affec

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GEL ELECTROPHORESIS

GEL ELECTROPHORESIS. Gel electrophoresis is a process used by scientists to separate mixtures of molecules by size, creating a DNA “fingerprint” It is most often used to separate molecules of proteins and molecules of DNA. Everyone has their own unique DNA (unless you have an identical twin)

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Gel electrophoresis

Gel electrophoresis. L Mathias. Definition. Separation of DNA fragments according to size, based on movement through a gel medium when an electric field is applied. . DNA negatively charged. DNA cut up using restriction endonucleases. Make up the gel which the DNA will be put into.

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Gel Electrophoresis

Gel Electrophoresis. The questions. Why? To separate pieces of DNA by size. How? Use electrical charge to pull the negatively charged DNA through a gel that has small pores in it.

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Gel Electrophoresis

Gel Electrophoresis. Steps. Breakdown the protein into amino acids Break amide/peptide bond Called hydrolysis (opposite of condensation) Acid or base and heat required Add protein to gel containing a buffer Apply a voltage Amino acids move based on mass and charge Dye amino acids

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Gel Electrophoresis

Gel Electrophoresis. By: Tasnuva Jhileek Dr. Francine Norflus Biotechnology. What is Gel Electrophoresis?. A procedure that separates molecules based on size and charge. Uses an electric field Molecules move through gel made of agar or polyacrylamide. Simple Procedure.

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Gel Electrophoresis

Gel Electrophoresis. First, What……. Simply put, gel electrophoresis is a technique used to separate molecules such a DNA, RNA and proteins according to size. Requires an electric current as these molecules are negatively charged

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Gel Electrophoresis

Gel Electrophoresis. Purpose of Gel Electrophoresis. A method for separating DNA Can be used to separate the size of DNA RNA Protein We will be using it to separate DNA. DNA. What you start with: A variety of different fragments of DNA all mixed together

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Gel Electrophoresis

Gel Electrophoresis. Resource: http://web.utk.edu/~khughes/GEL/sld001.htm. What is Gel Electrophoresis?. Gel Electrophoresis separates molecules on the basis of their charge and size . The charged macromolecules migrate across a span of gel because they are placed in an electrical field .

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Gel Electrophoresis

Gel Electrophoresis. Measurement of DNA Concentration. Agarose Gel Electrophoresis ( Bases for DNA separation) Agarose melted in a buffer and poured into a horizontal tray When solidified, the gel contains small holes or pores through which the DNA fragments will travel

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Gel Electrophoresis

Gel Electrophoresis. What is Gel Electropho.

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Gel Electrophoresis

Gel Electrophoresis. Gel electrophoresis is a method that separates macromolecules, either nucleic acids or protein. Electrophoresis describes the migration of charged particles under the influence of an electric field. A gel is a colloid in a solid form.

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Part 1. Gel electrophoresis

Part 1. Gel electrophoresis. DNA is negatively charged (because of phosphate backbone) DNA will be attracted to positively charged poles and repelled from negatively charged ones. Part 2. Restriction Endonucleases (aka restriction enzymes). Enzymes that “cut” DNA in a sequence-specific manner

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Gel Electrophoresis

Gel Electrophoresis. Digital image of 3 plasmid restriction digests run on a 1% w/v agarose gel, stained with ethidium bromide. The DNA size marker is a commercial 1 kbp ladder. The position of the wells and direction of DNA migration is noted.

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GEL ELECTROPHORESIS:

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Gel Electrophoresis

Gel Electrophoresis. A molecular biology tool. Purpose . To separate and analyze/compare fragments of DNA. 4 Main Steps:. 1) Restriction Enzymes: Either one or several R.E.’s is added to a sample of DNA. Enzymes cut them into fragments. 2) Prepare Gel:

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Gel Electrophoresis

Gel Electrophoresis. By: Brian Barnes, Gary Pope, Eliza Morgan. What is GE?. Pronounced Gel ee-LEK-tro-fo-REE-sis Technique for separating DNA or Protein Molecules into strands according to length. What’s The Point?. Used to separate DNA and identify strands of interest

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GEL ELECTROPHORESIS

BIOCHEMISTRY

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Gel Electrophoresis

Gel Electrophoresis. Student Instructions Edvotek set up. Salk Institute Mobile Lab. Gel Electrophoresis Student Station Set-up. tip container w/tips. waste container. 5 µ l fixed pipettor. practice sheet in protector. foam epi rack w/ 1 food color tube each: orange, green, purple.

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Gel Electrophoresis

Gel Electrophoresis. Student Instructions Edvotek set up. Salk Institute Mobile Lab. Gel Electrophoresis Student Station Set-up. tip container w/tips. waste container. micropipettor. practice sheet in protector. foam epi rack w/ 1 food color tube each: orange, green, purple.

709 views • 8 slides

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