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Eluent :(A); 20mM Sodium phosphate buffer (pH7.0) (B);(A) + 0.5M NaCl (pH7.0) Linear gradient:(A) to (B), 10min Flow rate :1.5mL/min. IEC SP-420N.
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Eluent :(A); 20mM Sodium phosphate buffer (pH7.0) (B);(A) + 0.5M NaCl (pH7.0) Linear gradient:(A) to (B), 10min Flow rate :1.5mL/min IEC SP-420N