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Explore features like MA plot, linear fit normalization, gene selection, and K-Means clustering for differential gene expression. Identify up and down-regulated genes in hepatoma patients through hierarchical clustering with most discriminative genes.
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C5: Software for Microarray Analysis高成炎、黃瑞仁、張春梵、陳朝欽 (November/2005)
MA-Plot and Other Program Listing • compustt spot features • maplot matlab MA plot • linearfit normalization • geneUD up/down genes • cluster K-means Algo. • bestBC feature selection • select gene selection • listgene gene listing • denC.m Dendrogram
Up Regulated Genes BC007058, X01098 AI133162, K02922 L32179, AL532086 X06290, BC008983 Down Expressed Genes AI133196, BG766355 M24173, S80335 The up-regulated (down-regulated) genes are defined as those genes with the normalized log2 ratio log2(Cy3/Cy5) greater (smaller) than a user-specified threshold T (-T), e.g., T=2.5 Differentially Expressed Genes Selected from 55 Hepatoma Patients
Hierarchical Clustering on 50 Patients of Hepatoma with 29 HBV and 21 HCV Using 26 Most Discriminative Genes