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Expression microarray analysis of NB cell lines treated with “epigenetic drugs”: with 5-Aza-dC: SK-N-AS, SK-N-DZ and IMR-32 with 5-Aza-dC/TSA: SK-N-AS, SK-N-BE(2), SK-N-DZ and IMR-32 Additional file 1, 2, 3, 4. Methylation microarray analysis using Illumina 27K arrays
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Expression microarray analysis of NB cell lines treated with “epigenetic drugs”: • with 5-Aza-dC: SK-N-AS, SK-N-DZ and IMR-32 • with 5-Aza-dC/TSA: SK-N-AS, SK-N-BE(2), SK-N-DZ and IMR-32 • Additional file 1, 2, 3, 4 • Methylation microarray analysis using Illumina 27K arrays • 59 primary NB tumors • 4 NB cell lines (SK-N-AS, SK-N-BE(2), SK-N-DZ and IMR-32) • Adrenal • Additional file 2 8 candidate genes chosen Additional file 5 Differential methylation analysis (using 27K data) Figure 3, Figure 4 • qRT-PCR analysis of NB cell lines treated with “epigenetic drugs”: • 9 NB cell lines • Additional file 6 • BSP or MSP analysis: • 9 NB cell lines • 13-24 primary NB tumors • Adrenal • Healthy blood controls • Figure 2 • qRT-PCR analysis: • 43 primary NB tumors • 9 healthy control tissues • Additional file 7 3 genes with differential methylation in favorable vs unfavorable outcome: KRT19, PRKCDBP, SCNN1A Figure 3, Figure 4 3 genes with differential expression in favorable vs unfavorable outcome: KRT19, PRKCDBP, DUSP23 Additional file 7
