1 / 39

MODELLING PROTEOMES RAM SAMUDRALA ASSOCIATE PROFESSOR UNIVERSITY OF WASHINGTON

Explore how an organism's genome specifies behavior and characteristics through proteome analysis. Discover thousands of sequences, structural folds, functions, and expression patterns, all crucial for biological interactions and protein folding. Learn about de novo modeling, protein structure, and interaction predictions for insights into various biological systems.

htyler
Download Presentation

MODELLING PROTEOMES RAM SAMUDRALA ASSOCIATE PROFESSOR UNIVERSITY OF WASHINGTON

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. MODELLING PROTEOMES RAM SAMUDRALA ASSOCIATE PROFESSOR UNIVERSITY OF WASHINGTON How does the genome of an organism specify its behaviour and characteristics?

  2. PROTEOME ~60,000 in human ~60,000 in rice ~4500 in bacteria Several thousand distinct sequence families

  3. STRUCTURE A few thousand distinct structural folds

  4. FUNCTION Tens of thousands of functions

  5. EXPRESSION Different expression patterns based on time and location

  6. INTERACTION Interaction and expression are interdependent with structure and function

  7. PROTEIN FOLDING Protein sequence …-L-K-E-G-V-S-K-D-… One amino acid Unfolded protein Spontaneous self-organisation (~1 second) Native biologically relevant state Gene …-CTA-AAA-GAA-GGT-GTT-AGC-AAG-GTT-… • Not unique • Mobile • Inactive • Expanded • Irregular

  8. PROTEIN FOLDING Spontaneous self-organisation (~1 second) Native biologically relevant state Gene …-CTA-AAA-GAA-GGT-GTT-AGC-AAG-GTT-… Protein sequence …-L-K-E-G-V-S-K-D-… One amino acid Unfolded protein • Not unique • Mobile • Inactive • Expanded • Irregular • Unique shape • Precisely ordered • Stable/functional • Globular/compact • Helices and sheets

  9. STRUCTURE One distance constraint for every six residues One distance constraint for every ten residues 0 2 4 6 ACCURACY Experiment (X-ray, NMR) Computation (de novo) Computation (template-based) Hybrid (Iterative Bayesian interpretation of noisy NMR data with structure simulations) Cα RMSD

  10. DE NOVO MODELLING Sample conformational space such that native-like conformations are found SELECT Hard to design functions that are not fooled by non-native conformations (“decoys”) Astronomically large number of conformations 5 states/100 residues = 5100 = 1070

  11. DE NOVO MODELLING Make random moves to optimise what is observed in known structures GENERATE … … Find the most protein-like structures MINIMISE … … Filter based on all-atom pairwise interactions, bad contacts compactness, secondary structure, consensus of generated conformations FILTER

  12. TEMPLATE-BASED MODELLING SCAN ALIGN KDHPFGFAVPTKNPDGTMNLMNWECAIP KDPPAGIGAPQDN----QNIMLWNAVIP ** * * * * * * * ** … … Build initial models from multiple templates using minimum perturbation Construct nonconserved side and main chains using graph theory and semfold Refine using constraints derived from multiple templates

  13. STRUCTURE T0290 – peptidyl-prolyl isomerase from H. sapiens T0288 – PRKCA-binding from H. sapiens 2.2 Å Cα RMSD for 93 residues (25% identity) 0.5 Å Cα RMSD for 173 residues (60% identity) T0332 – methyltransferase from H. sapiens T0364 – hypothetical from P. putida 2.0 Å Cα RMSD for 159 residues (23% identity) 5.3 Å Cα RMSD for 153 residues (11% identity) Liu/Hong-Hung/Ngan

  14. HYBRID MODELLING http://protinfo.compbio.washington.edu/protinfo_nmr http://protinfo.compbio.washington.edu/psicsi Hong-Hung

  15. FUNCTION Ion binding energy prediction with a correlation of 0.7 Calcium ions predicted to < 0.05 Å RMSD in 130 cases Meta-functional signature accuracy Meta-functional signature for DXS model from M. tuberculosis Wang/Cheng

  16. INTERACTION Transcription factor bound to DNA promoter regulog model from S. cerevisiae Prediction of binding energies of HIV protease mutants and inhibitors using docking with dynamics BtubA/BtubBinterolog model from P. dejongeii (35% identity to eukaryotic tubulins) McDermott/Wichadakul/Staley/Horst/Manocheewa/Jenwitheesuk/Bernard

  17. SYSTEMS Example predicted protein interaction network from M. tuberculosis (107 proteins with 762 unique interactions) Proteins PPIs TRIs H. sapiens 26,741 17,652 828,807 1,045,622 S. cerevisiae 5,801 5,175 192,505 2,456 O.sativa (6) 125,568 19,810 338,783 439,990 E. coli 4,208 885 1,980 54,619 In sum, we can predict functions for more than 50% of a proteome, approximately ten million protein-protein and protein-DNA interactions with an expected accuracy of 50%. Utility in identifying function, essential proteins, and host pathogen interactions McDermott/Wichadakul

  18. SYSTEMS Combining protein-protein and protein-DNA interaction networks to determine regulatory circuits McDermott/Wichadakul

  19. INFRASTRUCTURE ~500,000 molecules over 50+proteomes served using a 1.2 TB PostgreSQL database and a sophisticated AJAX webapplication and XML-RPC API http://bioverse.compbio.washington.edu http://protinfo.compbio.washington.edu Guerquin/Frazier

  20. INFRASTRUCTURE Guerquin/Frazier

  21. INFRASTRUCTURE http://bioverse.compbio.washington.edu/integrator Chang/Rashid

  22. APPLICATION: DRUG DISCOVERY CMV KHSV HSV Jenwitheesuk

  23. APPLICATION: DRUG DISCOVERY Computionally predicted broad spectrum human herpesvirus protease inhibitors is effective in vitro against members from all three classes and is comparable or better than anti-herpes drugs CMV HSV KHSV Our protease inhibitor acts synergistically with acylovir (a nucleoside analogue that inhibits replication) and it is less likely to lead to resistant strains compared to acylovir HSV HSV Lagunoff

  24. APPLICATION: NANOTECHNOLOGY Oren/Sarikaya/Tamerler

  25. FUTURE + + Computational biology Structural genomics Functional genomics MODELLING PROTEIN AND PROTEOME STRUCTURE FUNCTION AT THE ATOMIC LEVEL IS NECESSARY TO UNDERSTAND THE RELATIONSHIPS BETWEEN SINGLE MOLECULES, SYSTEMS, PATHWAYS, CELLS, AND ORGANISMS

  26. ACKNOWLEDGEMENTS Current group members: Past group members: Collaborators: • Baishali Chanda • Brady Bernard • Chuck Mader • David Nickle • Ersin Emre Oren • Ekachai Jenwitheesuk • Gong Cheng • Imran Rashid • Jeremy Horst • Ling-Hong Hung • Michal Guerquin • Rob Brasier • Rosalia Tungaraza • Shing-Chung Ngan • Siriphan Manocheewa • Somsak Phattarasukol • Stewart Moughon • Tianyun Liu • Vania Wang • Weerayuth Kittichotirat • Zach Frazier • Kristina Montgomery, Program Manager • Aaron Chang • Duncan Milburn • Jason McDermott • Kai Wang • Marissa LaMadrid • James Staley • Mehmet Sarikaya/Candan Tamerler • Michael Lagunoff • Roger Bumgarner • Wesley Van Voorhis Funding agencies: • National Institutes of Health • National Science Foundation • Searle Scholars Program • Puget Sound Partners in Global Health • UW Advanced Technology Initiative • Washington Research Foundation • UW TGIF

  27. MOTIVATION FOR DETERMINING PROTEIN STRUCTURE The functions necessary for life are undertaken by proteins. Protein function is mediated by protein three-dimensional structure. Knowing protein structure at high resolution will enable us to: Determine and understand molecular function. Understand substrate and ligand binding. Devise intelligent mutagenesis and biochemical experiments to understand biological function. Design therapeutics rationally. Design novel proteins. Knowing the structures of all proteins encoded by an organism’s genome will enable us to understand complex pathways and systems, and ultimately organismal behaviour and evolution. Applications in the area of medicine, nanotechnology, and biological computing.

  28. ALL-ATOM SCORING FUNCTION distance bins known structures atom-atom contacts AO AN AC … YOH 167 X167 contacts AO AN AC ... YOH AO AN AC … YOH s(dab) for contacts AO AN AC ... YOH candidate structure atom-atom contacts AO AN AC … YOH NxN contacts AO AN AC ... YOH

  29. CRITICAL ASSESSMENT OF STRUCTURE PREDICTION Pre-CASP CASP Bias towards known structures Blind prediction

  30. STRUCTURE TO FUNCTION? Hydrolase Ligase Lyase Oxidoreductase Transferase TIM barrel proteins 2000+ experimental structures

  31. INTEROLOG MODELLING Interacting protein database Target proteome 85% Protein a Protein A Experimentally determined interaction Predicted interaction Protein b Protein B 90% Assign confidence based on similarity and strength of interaction Paradigm is the use of homology to transfer information across organisms; not limited to yeast, fly, and worm Consensus of interactions helps with confidence assignments

  32. E. coli INTERACTIONS McDermott

  33. M. tuberculosisINTERACTIONS McDermott

  34. C. elegans INTERACTIONS McDermott

  35. H. sapiens INTERACTIONS McDermott

  36. Network-based annotation for C. elegans McDermott

  37. KEY PROTEINS IN ANTHRAX Articulation points McDermott

  38. HOST PATHOGEN INTERACTIONS McDermott

More Related