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MHC Tetramer Technology: Recent Developments

MHC Tetramer Technology: Recent Developments. John D. Altman – Emory University. Recent Developments in MHC Tetramer Technology. Contract Renewed for 7 years New website Production of class I MHCp by peptide exchange High-throughput tetramers (HTT)

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MHC Tetramer Technology: Recent Developments

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  1. MHC Tetramer Technology: Recent Developments John D. Altman – Emory University

  2. Recent Developments in MHC Tetramer Technology • Contract • Renewed for 7 years • New website • Production of class I MHCp by peptide exchange • High-throughput tetramers (HTT) • Class II – summary of available alleles and technology • Fluorophores • CyTOF tetramers • StvC and StvC6

  3. Exchange-competent class I proteins made in 293T cells

  4. Exchange-competent Class I MHC tetramers for multiple alleles

  5. Class II allele table

  6. Brilliant Violet 421 – A Bright New Fluorophore for Tetramers PE Summary of Fluorophores Used for Tetramers BV421 mCD1d PBS57 tetramer as shown Pacific Blue CD3 FITC

  7. Tetramers for CyTOF Cys(1 or 6) Cys(1 or 6) Cys(1 or 6) Cys(1 or 6) Newell et al. Immunity. 36, 142-52 2012 PMID: 22265676

  8. Acknowledgments • John Altman • Rick Willis • John Shires • VasanthiGovindu • Chris Ibegbu

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