The New age Shower

1. The New age Shower James Diewald Michelle Cisar Chris Jones Paul Georgadis

2. The Experiment • Bacteria are everywhere. • After collecting and identifying bacteria collected from the entire western campus we decided to narrow our search to one bathroom. • That bathroom is 3 South Peabody Hall. • Samples will be taken from designated sights in the bathroom.

3. The Essential Background • Most domestic bacteria ar harmless but some rare strains can cause death. • Bacteria are the most common organisms on earth. • Bacteria are 80% water. • Every one of you has bacteria inside of you • Bacteria prosper in warm moist places, preferably between 25 and 35 degrees C (your body temperature). • Your bacteria are good for you. • High volume bathrooms are bacteria breeding grounds.

4. The decided sampling locations are as follows. 1. The toilet seat, flush handle and outgoing door latch. 2. The outgoing door handle. 3. The sink bowl and knobs. 4. The shower floor and knobs. We expect a number of bacteria to be thriving at these prescribed locations. All of which are thought to be infectious to humans and some may be deadly. What you Need to Know

5. The Suspects • The cell wall is composed of peptidoglycan, a protein-sugar molecule. • No conventional nucleus. • Uses flagellum for movement.

6. The Suspects (1) Staphylococci -yellow, spherical cells in irregular clusters -carried by 15-20% of population -common infections: hair follicle, bone, intestine, urinary tract

7. The Suspects (2) Streptococci -small white, semi-transparent, chains of spherical cells -very common -common infections: throat, skin, joints, heart

8. The Suspects (3) E-coli -smooth, orange, spherical cells -part of normal flora of intestinal tract -common infections: intestines, traveler’s diarrhea, fatal cholera-like disease

9. The Suspects (4) Salmonella -larger, tan, smooth cells -transmitted through contaminated food and water -common infections: gastrointestinal tract, abdomen

10. The Suspects (5) Campylobactor -white, curved rods -ranks with E-coli as major cause of diarrhea in world -common infections: intestinal tract, diarrhea

11. We expect the shower and the door handle to have the highest levels of bacteria. The shower bacteria levels will be high due to traffic and moisture levels that advocate bacteria growth. The door handle is the single most touched object in the bathroom and may therefore have high levels of bacteria. We also expect a significant difference before and after cleaning. Further Expectations

12. Materials • 16 Agar Plates • Cotton Swabs • Permanent Marker • Tape (to seal agar after sampling)

13. We took before and after cleaning samples on each of two days from the named locations. A specific “Circle X” plating method was created in order to insure a uniform bacteria distribution on the agar plates. To calculate the percent coverage of each species, we utilized Photoshop to lay a grid over a series of digital photos. The photo was zoomed in on and the percentage was established. Sampling Procedures

14. Mission Results • As before, bacteria are everywhere. • The following graphs will provide detailed data on our results.

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22. Final Conclusions • Statistical analysis via Statview showed no significant difference before and after cleaning. • Our graphs did show otherwise. • We assume that a larger data set would yeild our predicted results.