Study Guide to Help with Laboratory Exam 1

1. Study Guide to Help with Laboratory Exam 1 Microbiology 1214 A partial study guide only. Not intended to replace the review sheets or the “Know the following” sections on your labs. These slides are not presented in any particular order.

2. Name this Procedure

3. Identify the two types of bacteria present by shape and gram stain. In a gram stain what is the primary stain? The mordant? The counterstain? How does this differ from a simple stain?

4. Identify the shape and gram stain of the bacteria present in this gram stain.

5. Identify the shape and gram stain of the bacteria pictured. Think on this one!

6. Name the gram stain and shape of the bacteria pictured.

7. Name the genus of the organism pictured. Note the size when determining the organism.

8. Identify this type of agar. We use this agar to check for _______.

9. Name This Technique. What is its purpose?

10. What type of hemolysis is pictured below?

11. Identify the Following I go straight from the 4x objective to the 100 x objective? Why?

12. Identify the Following

13. Identify the staining procedure pictured.

14. Identify what we are doing here. What type of plate would we be making using this technique?

15. Name the Structure Pictured. What Settings Do We Use to Sterilize Bacteria Using this Instrument? What temperature do we use to sterilize? At what temperature does agar melt? At what temperature does agar solidify? At what temperature do we prefer to work with liquified agar in the lab?

16. Name this Technique

17. Name this Organism. How does it reproduce? What is another name for a bud?

18. In the capsule stain, what is the primary stain? What is used to stain the background? What is the method used to make the smear called? What is an advantage to not heat fixing? What is a disadvantage to not heat fixing?

19. In the capsule stain, what is the acidic dye used in the procedure? • What is the basic dye used in the procedure.

20. Identify the three types of flagella pictured. What is the difference between true bacterial movement and brownian movement?

21. The pictured bacteria is 1 micrometer in length. How many lines would that be on the on the ocular micrometer if I am using the oil objective?

22. Pictured are endospores. What two techniques do we use to view endospores. What dye(s) would we use in each technique. What shape would we use to classify the structure?

23. In an acid fast stain, what is the primary dye? Decolorizer? Counterstain? What is the waxy-like lipid material present in the cell wall? Name two diseases caused by an acid fast bacterium. What does the term “acid” refer to in acid fast bacteria? Know the steps of this staining procedure, know the stains used in each step.

24. Name the staining technique pictured. This is a negative stain. What is another name for negative stain? Name two dyes that could be used for this procedure. What is stained in this procedure?

25. What size is the bacillus pictured above?

26. What is the name of the chamber pictured below? We used the genus Saccharomyces to do this count. Saccharomyces is a fungus, what media (media of choice) would we choose to plate Saccharomyces out on?

27. What do we call this procedure? When is it used? If I counted 56 bacteria on the 10-7 plate, how many bacteria would be in my original stock solution? On the 10-5 plate?

28. What is the correct term to refer to each of the growths we see on this plate? If I wanted to count this I might place it on a source with a light called a _______ Counting Chamber.

29. How many squares would I count?

30. Identify the plate below that is countable? What are the reference numbers associated with a countable plate? A B C

31. A sample of E.coli is diluted according to the above diagram. The number of colonies that grew is indicated on the petri plates. Using the countable plate determine how many CFUs (colony forming units) are there per ml in the flask of the original sample

32. A sample of E.coli is diluted according to the above diagram. The number of colonies that grew is indicated on the petri plates. Using the countable plate, determine how many CFUs are there per ml in the flask of original sample?

33. This plate was made from a swab obtained from the bottom of a shoe. Can you differentiate between bacteria and fungi? How do we tell the two apart?

34. This technique is performed after my smear has dried. What is the name of this technique? Give two reasons why it is performed?

35. If I am doing a gram stain and leave out the mordant, what color will all of my bacteria end up being?

36. Identify this stain procedure. What are we looking for with this stain? The presence of a capsule increases the __________ of the bacterium. What composes a capsule?

37. What staining procedure is this? What are the green structures?

38. What type flagella is pictured?

39. Why do we use oil on the oil immersion objective? Because it has the same ______________________________ as glass.

40. What is the proper method of storing a petri dish in an incubator. Give two reasons why it is important to store by this method.?

41. By the way: • Name some acid dyes, some basic dyes. • An acid dye stains the _____ while a basic dye stains the _____. • Given a staining procedure be able to tell me whether a dye used in it is acidic or basic. • Name a gram negative bacteria studied in lab. • Name a gram positive bacteria studied in lab.

42. Bacteria or Fungus?

43. Identify the hemolysis indicated by the arrow.

44. Identify the hemolysis at the arrows.

45. Identify the shape of the bacteria pictured at the arrows. (It isn’t bacillus!)

46. Assuming we are on oil, what is the size of one coccus of the chain?