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Confirmation of Two SMV Resistance Genes in J05 Soybean Using SSR Markers

Confirmation of Two SMV Resistance Genes in J05 Soybean Using SSR Markers. Ainong Shi, Pengyin Chen, Cuiming Zheng, Innan Martinez, Bo Zhang, Anfu Hou, and Dexiao Li. University of Arkansas. Soybean Mosaic Virus (SMV). SMV is the most destructive viral disease in soybean.

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Confirmation of Two SMV Resistance Genes in J05 Soybean Using SSR Markers

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  1. Confirmation of Two SMV Resistance Genes in J05 Soybean Using SSR Markers Ainong Shi, Pengyin Chen, Cuiming Zheng, Innan Martinez, Bo Zhang, Anfu Hou, and Dexiao Li University of Arkansas

  2. Soybean Mosaic Virus (SMV) • SMV is the most destructive viral disease in soybean. • Seven SMV strains G1 to G7 were identified in U.S. • Genetic resistance is the primary method of controlling this disease. • Three loci, Rsv1, Rsv3, and Rsv4 have been identified.

  3. SMV Symptoms R N S R N S = Resistant = Necrosis = Susceptible (symptomless) (Mosaic)

  4. SMV Resistance Loci R N S R R R G1 G7 G1 G7 G1 G7 PI96983 (Rsv1) L29 (Rsv3) V94-5152 (Rsv4) SMV Strain G1 G7 Gene _________________________________________________________________________________________________________ PI 96983 R N Rsv1 ----------------------------------------------------------------------------------------------------------------------------- L29 S R Rsv3 --------------------------------------------------------------------------------------------------------------------------------------------------------- V94-5152 R R Rsv4

  5. R R G1 G7 J05 (Rsv1Rsv3) * Zheng et al. 2006. J. Hered. 97(5):429-437.

  6. Objectives • To confirm two genes, Rsv1Rsv3, in J05 • To identify SSR markers linked to the two genes in J05

  7. F2 population and F2:3 Lines for genetic analysis (S) (R) J05 x Essex (Rsv1Rsv1Rsv3Rsv3)(rsv1rsv1rsv3 rsv3) F1 (Rsv1rsv1Rsv3rsv3) F2 SMV strain G1 G7 Rsv1 R N Rsv3 S R X G1 3R : 1S (3RsvRsv1 : 1rsv1rsv1) G7 F2:3 1R : 2Seg : 1S (1Rsv3Rsv3 : 2Rsv3rsv3 : 1rsv3rsv3)

  8. Greenhouse Evaluation

  9. J05 x Essex F2

  10. Genetic Segregation in (J05 x Essex) F2 population SMV strain G1 R S Exp. c2 R-gene -------------------------------------------------- 102 36 3:1 0.087 Rsv1 R = Resistant (symptomless) S = Susceptible (mosaic)

  11. the G1-susceptible F2 plants (No Rsv1) SMV strain G1 G7 Rsv1 R N Rsv3 S R F2:3 lines G7 R Seg. S Exp. c2 R-gene -------------------------------------------------------- 10 20 15 1:2:1 0.087 Rsv3 R = Homozygous resistant, S = Homozygous susceptible, Seg. = Segregating

  12. Gore et al. 2002. Genome 45:592-599 Jeong et al. 2002. Crop Sci. 42:265-270. Cregan. 2003. (http://bldg6.arsusda.gov/~pooley/soy/cregan/cregan.htm)

  13. Genetic map of the Rsv1 region 59.60 Sat_297 62.79 Sat_229 63.69 Satt114 66.55 Sat_234 68.44 SOYHSP176 68.91 Sat_154 Rsv1 71.41 Satt510 72.97 Sat_317 74.13 Sct_033 MLG F

  14. An example of SSR primer Satt114 for Rsv1 J05 x Essex F2 population P1=J05, P2=Essex, R = resistant, and S = susceptible plants of reaction to SMV strain G1 in the F2.

  15. Satt114 3.8 cM Sat_154 0.5 cM Rsv1 2.3 cM Satt510 A genetic map for the Rsv1 region (MLG) F.

  16. 93.49 Satt063 Genetic maps of the Rsv3 region 97.92 Satt560 Rsv3 100.10 Sat_424 100.59 Satt726 113.61 Satt687 MLG B2

  17. Polymorphisms in J05 x Essex F2 plants amplified from Satt726 for Rsv3

  18. Rsv3 1.5 cM Sat_424 0.5 cM Satt726 A genetic map for the Rsv3 region on MLG B2

  19. SUMMARY • J05 is resistant to all seven U. S. SMV strains • The resistance to SMV strain G1 was controlled by Rsv1 on MLG F and to G7 by Rsv3 on MLG B2 in J05. • Two genetic maps were constructed for Rsv1 and Rsv3 regions in J05. Satt114 Rsv3 3.8 cM 1.5 cM Sat_154 0.5 cM Rsv1 Sat_424 2.3 cM 0.5 cM Satt726 Satt510 MLG B2 MLG F

  20. Thank you!

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