Biological Sciences 318- Parasitology Lab Platyhelminthes - Cestodes

1. Biological Sciences 318- Parasitology Lab Platyhelminthes - Cestodes

2. Kingdom: Animalia • (unranked): Protostomia • (unranked): Spiralia • (unranked): Platyzoa • Phylum:

3. Platyhelminthes Characteristics: 1) simplest bilaterally symmetrical animals 2) 3 layers fundamental cell layers (ectoderm, endoderm, mesoderm) 3) body contains no internal cavity 4) dorsoventrally flattened (diffusion) 5) somepossess a blind gut (i.e. it has a mouth but no anus) 6) protonephridial excretory organs (flame cell) 7) elaborate nervous system (allows for invasion of a variety of habitats) 8) reproduction mostly sexual as hermaphrodites 9) inability to synthesize fatty acids and sterols (commensals or parasites)

4. Cestoda Tapeworms • all endoparasitic (nearly every species of vertebrate) • no mouth, no digestive system (only vestigal sucker and pharynx remaining) • large reproductive system! • absorb nutrients through tegument

5. Eucestoda True tapeworms • Form and Function: a. Scolex with holdfast organelles (suckers, grooves, hooks, spines) • - 3 types of sucking depressions: • i- Bothria: shallow sucking grooves (Pseudophyllidea) • ii- Bothridia: 4 leaflike flexible structures (Tetraphyllidea) • iii- True suckers/acetabula (Cyclophyllidea) • b. Neck • c. Strobila (divided into proglottids) • Strobilation: Addition of new proglottids in neck region • Mature from AP • Apolysis: gravid proglottid breaks off releasing eggs • Alternatively eggs released through uterine pore.

6. Eucestoda True tapeworms Form and Function: Reproductive Systems Order: Cyclophyllidae (Taenia spp.); Pseudophyllidae (Diphyllobothrium latum)

7. Eucestoda True tapeworms • Form andFunction: Development • Two-phaselifecycles:  intermediate •  definitive • Embryogenesis within egg results in larva  Oncosphere • HatchingofOncosphere in intermediate host(extraintestinal site) • Metamorphosis into juvenile withscolex  Metacestode (1 tovarious different stages) • Development of adult tapeworm in definitive host(intestinal site)

8. Eucestoda True tapeworms • Order: Pseudophyllidae • bothriate scolex(possessing dorsal and ventral groove = bothrium) • Testes and vitellaria scattered throughout the proglottid • The ovary is bilobed and posterior.Genital pores ventral • An uterine pore present on dorsal or ventral surface • Eggs are operculate and contain ciliated embryo, the coracidium • Adult parasites of all classes of vertebrates, but fish are their primary host. • The life cycle involves procercoid (larval stage often found in copepods) and plerocercoid • larval stage (found in fish) •  Diphyllobothrium latum(broad fish tapeworm) • Can be a serious pathogen, causing a pernicious anemia in human due to the worm absorbing • large amounts of vitamin B12

9. Eucestoda True tapeworms • Order: Cyclophyllidea • scolex that usually contains four suckers; rostellum is present or absent (may be armed) • The genital pore is usually lateral; Vitelline gland is single, compact, and posterior to the ovary. • Eggs develop into some form of bladderworm in invertebrate or vertebrate intermediate host. • Common tapeworms of birds and mammals, although some are found in reptiles. • Infective eggs contain an oncosphere larva that bears 6 hooks. • Variety of intermediate host types, both invertebrate and vertebrate P A

10. Eucestoda True tapeworms • Order: Cyclophyllidea • Depending on cestode species: larval stage in the vertebrate intermediate host the • Metacestode: cysticercus, strobilocercus, coenurus (multiceps), hydatid or cysticercoid. • Family Taeniidae, Genus Echinococcus, Family Dilepididae, Hymenolepididae, Anoplocephalidae

11. Taenia pisiformis Hymenolepis diminuta cysterceroid life stage Hymenolepis diminuta egg Diphyllobothrium latum Taenia egg Echinococcus granulosus Diphyllobothriumltaum eggs Echinococcus granulosus hyatid cysts multilocular cyst Dipylidium caninum

12. Review SessionFri. Feb 6 1:00 – 4:00Mon. Feb. 16 2:00-5:00Lab ExamFeb 17-19(date varies by lab section)

13. Learning Objectives • 1. Platyhelmintes - Flatworms • Why are they flat? Why are they special? • Know that reproductive structures are used in id! • 2. Subclass Eucestoda • Polyzoic; body parts; Strobilation • Hermaphrodites or monoecious; compare eggs • Difference between Cyclo- & Pseudophyllidae • 3. Order Cyclophyllidae • General characteristics • 4. Family Taeniidae • General charcterristics • 5. Genus Taenia • Life cycle, hosts, stages, transmission, etc. • Taeniapisiformis(scolex, immature, mature, gravid proglottid) • Anatomy of mature proglottid • Taenia eggs: non-operculate, radial lines (striated appearance), thick-shelled • Cysticercus: cross section + whole mount; invaginatedscolex; • 6. Genus Echinococcus • Echinococcusgranulosus • Life cycle, host, transmission, pathology • Visual id • Tissue section: unilocular cyst, brood caspules, protoscolices • Hydatid sand: protoscolices • Echinococcusmultilocularis: multilocular cyst (Why worse?) • 7. Family Dilepididae • General characteristics • 8. Dipylidiumcaninum • Life cycle, hosts, transmission, etc. • Double-pored tapeworm! • Double reproductive system, egg capsules containing many eggs, scolex • 9. Family Hymenolepididae • Life cycle • Cysticercoid slide in invertebrate Hydra host • Eggs • 10. Order Pseudophyllidae • General characteristics • 11. Diphyllobothriumlatum • Life cycle, transmission, host, stages, etc. • Pathology • Scolex: bothria • Eggs: operculate, contain ciliated embryo (coracidium) Vocabulary Monoecious Dioecious Endoparasitic Polyzoic Scolex Strobila Proglottids Operculum Coracidium Strobillation Metacestode Cysticercus Coenurus Hydatid cyst and sand Cysticercoid Evagination Bothria Vitellaria Gravid Brood capsules Unilocular cyst Multilocular cyst Protoscolex Procercoid Plerocercoid