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The Role of Estrogens in the Hippocampal-Neuropeptide Y

The Role of Estrogens in the Hippocampal-Neuropeptide Y. Damaly Ferreira Jana Veli s kov a Albert Einstein College of Medicine. Keywords. Estrogen- female sex hormones that are secreted by the ovary and responsible for typical female characteristics.

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The Role of Estrogens in the Hippocampal-Neuropeptide Y

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  1. The Role of Estrogens in the Hippocampal-Neuropeptide Y Damaly Ferreira Jana Veliskova Albert Einstein College of Medicine

  2. Keywords • Estrogen- female sex hormones that are secreted by the ovary and responsible for typical female characteristics. • Seizure- a sudden burst of electric activity in the brain • Epilepsy- a seizure disorder • Hippocampus- a part of the brain affected by estrogen and is associated with transferring information into memory • NPY- a powerful inhibitory system in the hippocampal hilar region implicated in the regulation of seizure activity. • Status Epilepticus- an episode or continuous seizure of 30 minutes or more

  3. Introduction • Objective: In our experiment, our goal is to figure out the role estrogens play in the Hippocampus- Neuropeptide Y region (NPY). • Hypothesis: Estrogens are accountable for the actions produced in the NPY. • Literature Review: In past studies, there have been human and animal studies that have created a lot of controversial data that debate the role of estrogens with seizures.

  4. Materials and Methods • Ovariectomy • Treat Sprague Dawley rats with PPT • PPT • 4,4,4-(4-propy-[1H]-pyrazole-1,3,5-triyl)trisphenol • ER beta and ER alpha agonist • C24H22N2O3  H2O • Perfusion • Brain is taken out of skull • Brain is immersed into 2-methylbutane, then stored into storage freezer • Washed with PBS (saline solution)

  5. Materials and Methods con’t • Immunohistochemistry • 50 µl of H2O2 + 5 ml of PBS • Wash wells with PBS (5 minutes each) • 160 µl of BSA + 5 ml of PBS + 20 µl Triton into wells for 90 minutes • Apply NPY antibody + 5 ml PBS for 90 minutes • Put 200 µl of solution in each well • Take out NPY antibody solution and wash 3 times with PBS

  6. Materials and Methods con’t • Immunohistochemistry con’t • 100 µl of BSA + 5 ml PBS + 25 µl anti-rabbit in wells for 1 hr • Wash 3 times with PBS • Apply AB in wells and settle for 1 hr • Wash 3 times with PBS • DAB

  7. Expected Results • The PPT used, will hopefully detect which Estrogen Receptor was triggered. • In the maps of the slides, we will hopefully see whether the Estrogen created any damage to the hippocampal area, or whether the NPY protected the hippocampal area from any damage.

  8. References • Immunohistochemistry con’t • Are the role of Estrogens in Seizures and Epilepsy: the bad guys or the good guys? 2005, Elsevier Ltd, Bronx, NY. • Carol Camfield, M.D 2004. What is epilepsy? http://www.epilepsy.com/101/ep101_epilepsy.html, 2 Aug 2006 • Centre for Cancer Education [on-line] 1997. http://cancerweb.ncl.ac.uk/cgi-bin/omd?query=seizure&action=Search+OMD, 3 Aug 2006. • Centre for Cancer Education [on-line] 1998. http://cancerweb.ncl.ac.uk/cgi-bin/omd?query=status+epilepticus, 2 Aug 2006. • The American Heritage® Dictionary of the English Language [on-line] 2000. http://dictionary.reference.com/browse/estrogen, 2 Aug 2006. • Veliskova, J., Velisek, L., Galanopoulou, A.S. and Sperber, E.F.: Neuroprotective effects of estrogens on hippocampal cells in adult female rats following status epilepticus. Epilepsia, 41 Suppl.6:S30-35,(2000).

  9. Acknowledgements • Jana Veliskova, M.D., Ph.D. • Hong Wong • Zunju • Tamara Dennis • Dr. Sat Bhattacharya • Harlem Children Society • MSKCC • The Audience

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