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MALDI demonstration

Frímann, Helgi, Long, Nanna. MALDI demonstration. Sample preperation. The sample of interest and matrix are dissolved in a volatile solvent The sample/matrix/solvent mixture is then spotted on a metal plate When the solvent has evaporated you can put your plate in to the vacuum

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MALDI demonstration

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  1. Frímann, Helgi, Long, Nanna MALDI demonstration

  2. Sample preperation • The sample of interest and matrix are dissolved in a volatile solvent • The sample/matrix/solvent mixture is then spotted on a metal plate • When the solvent has evaporated you can put your plate in to the vacuum • Anchor chips have hydrophilic area surrounded by hydrophobic ring

  3. Matrix Assisted Laser Desorption/ Ionization • Introduced by M. Karas and F. Hillenkamp in 1985 [1] • Mainly used for large molecules • Soft ionization • The vibrational energy added to the analyte molecule is about 4eV [2] • The ions formed are typically: [M+H]+, [M+Na]+, [M+K]+, [M+NH4]+ or [M-H]- • Ionization mechanism is not fully understood

  4. TOF mass spectrometer • A TOF MS is usually (always?) used with MALDI • Reflectron TOF • MSMS • Microchannel plate electron multiplier (10000 electrons for each ion) • MALDI-TOF “records”: ~300.000 amu, attomoles of material

  5. Electron multiplier

  6. Measurements • The data we have is from the protein BSA (Bovine serum albumin) • We took linear spectra in the demostration • Got the data from the peak at 927amu in MSMS mode

  7. References • [1] Karas, M.; Bachmann, D.; Hillenkamp, F. (1985). "Influence of the Wavelength in High-Irradiance Ultraviolet Laser Desorption Mass Spectrometry of Organic Molecules".Anal Chem 57: 2935–9 • [2] I. Bald, J. Kopyra and E. Illenberger, Angewandte Chemie-International Edition 45 (29), 4851-4855 (2006).

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