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Retrieved from: http://www.eng.uci.edu/files/images/gallery/Protein_Nanoparticle_Structure.jpg. Enhancing Antibacterial Efficacy using Protein Nanoparticles. Leslie Tan Zheng Yu Tan Jing Chong Erik Warnquist Varun Kulkarni. Introduction.
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Retrieved from: http://www.eng.uci.edu/files/images/gallery/Protein_Nanoparticle_Structure.jpg Enhancing Antibacterial Efficacy using Protein Nanoparticles Leslie Tan Zheng Yu Tan Jing Chong Erik Warnquist VarunKulkarni
Introduction • Pesticides are used to eradicate Agrobacterium tumefaciens • High percentage of pesticide does not reach the target species. • Result in water and soil pollution. • Threatens biodiversity.
Introduction • Usage of nanoparticles as drug carrier for pesticides • Increase in therapeutic efficacy • Increasing localisation to diseased sites • Decrease in side effect • Protein Nanoparticle are biodegradable, metabolisable and non-antigenic • Does not accumulate in tissue
Objective • To compare the effectiveness of antibiotic loaded albumin nanodroplets against antibiotic loaded albumin nanofibre on A. tumefaciens, grown both in vitro and in vivo.
Hypothesis • The two delivery techniques will be comparable, through both qualitative and quantitative means
Equipment • Electrospinning apparatus • Scanning electron microscope (SEM) • Homogenizer • Incubator • Environmental chamber • Spectrophotometer
Materials • Bovine Serum Albumin • Alcohol • A. tumefacians • Potato strips • Diffusion assays • tetracycline and ampicillin
Antibiotic loading - nanodroplets • Incubating nanoparticles in antibiotic solution • Antibiotic contained in nanoparticles • Done at protein's isoelectric point • Minimum solubility and maximum absorption • BSA: pH of 4.4 • Larger amount of antibiotic loaded • Antibiotic entrapment efficacy measured
Antibiotic loading - nanofibres • Antibiotics mixed in albumin solution • Homogenous solution • Hypothesis that spinning solution will result in the non polymer antibiotics also being spun
Effectiveness of antibiotic-loaded nanoparticles • Protein nanoparticles digested by proteases to release antibiotics • Antibiotic-loaded nanoparticles are subjected to: • A.tumefacians agar plates discs • A.tumefacians-potato strips
References • Buschle-Diller, G., Cooper, J., Xie, Z., Wu, Y., Waldrup, J., & Ren, X. (2007). Release of antibiotics from electrospunbicomponent fibers. Cellulose, 14(6), 553- 562 • Collins, A. (2001). Agrobacterium tumefaciens. Department of Plant Pathology, University of North Carolina State. Retrieved September 19, 2010 from: http:/www.cals.ncsu.edu/course/pp728/ Agrobacterium/Alyssa_Collins_profile.htm
Frenot, A., & Chronakis, I.S. (2003). Polymer nanofibers assembled by electrospinning. Current Opinion in Colloid and Interface Science, 8(1), 64-75. • Hyuk, Y.S., Taek, G.K., & Park, T.G. (2009). Surface-functionalized electrospunnanofibers for tissue engineering and drug delivery. Advanced Drug Delivery Reviews, 61(12), 1033-1042. • Jahanshahi, M. & Babaei, Z. (2008). Protein nanoparticle: A unique system as drug delivery vehicles. African Journal of Biotechnology, 7(25), 4926-4934.
Knee, M., & Nameth, S. (2007). Horticulture and Crop Science: Bacteria. The Ohio State University, Horticulture Department. Retrieved September 12, 2010 from : http://www.hcs.ohio- state.edu/hcs300/bact.htm • Kratz, F. (2008). Albumin as a drug carrier: Design of prodrugs, drug conjugates and nanoparticles. Journal of Controlled Release, 132(3), 171-183. • McManus, P. (2007). Antibiotic Use in Plant Disease Control. Fruit Pathology: University of Wisconsin-Madison. Retrieved September 13, 2010 from: http://www.plantpath.wisc.edu/fpath /antibiotic-use.htm
M.R., Jahanshahi, M., & Najafpour, G.D. (2006). Production of biological nanoparticles from bovine serum albumin for drug delivery. African Journal of Biotechnology, 5(20), 1918- 1923.