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Species: Leptodea fragilis (wetlands/river mouths)

Genetic Sampling. Species: Leptodea fragilis (wetlands/river mouths) Pyganodon grandis (wetlands/ nearshore/tribs – Lake Erie) Quadrula quadrula (wetlands/river mouths – Lake Erie) Lampsilis siliquoidea/radiata (Lake St. Clair drainage only)

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Species: Leptodea fragilis (wetlands/river mouths)

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  1. Genetic Sampling • Species: • Leptodeafragilis (wetlands/river mouths) • Pyganodongrandis (wetlands/nearshore/tribs – Lake Erie) • Quadrulaquadrula (wetlands/river mouths – Lake Erie) • Lampsilissiliquoidea/radiata (Lake St. Clair drainage only) • Ligumianasuta (where it can be found 1-2 swab samples/refuge) • Numbers (site = refuge scale): • Microsats – clips only: • Q. quadrula(at least 10, but <50/site) • L. fragilis(at least 10, but <50/site) • L. siliquoidea(at least 10, but <50/site) • mtDNA (m/f lineage) – sacrifice animals • P. grandis (15 males/site) • L. fragilis (15 males/site, subsampled from above) – juveniles? • Swabs only (endangered species) • L. nasuta (1-2 swabs/site)

  2. Clipping instructions (more at methods meeting): • pry open shell with blunt end of scalpel/spatula (no blade!) • use thumb or end of pencil or small rubber stopper to wedge open shell • clip ~0.5 cm2 piece of mantle tissue from shell edge using surgical scissors (from basic dissection kit – keep them sharp) • clip from ventral-anterior side (avoid siphons) • Swab samples • as above, but sterile swab 2-3 times along foot – avoid touch mantle and siphons

  3. Logistics • Zanatta lab: • 2 grad students + 2 undergrads • 2 teams will join field teams as dedicated clippers/swabbers • Krebs lab: • Trevor Prescott + undergrad will assist in clipping • 3 genetics teams to assist other with general field work and will do clipping in field

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