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Two countries, one goal, joint success!

Essential and toxic microelements in grains of Fusarium -infected maize and wheat genotypes: ICP-MS analysis. Zsuzsanna Kolbert, Attila Ördög, Ágnes Vashegyi, László Erdei. Two countries, one goal, joint success!. Materials and methods. Plant material :

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Two countries, one goal, joint success!

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  1. Essential and toxic microelements in grains of Fusarium-infected maize and wheat genotypes: ICP-MS analysis Zsuzsanna Kolbert, Attila Ördög, Ágnes Vashegyi, László Erdei Two countries, one goal, joint success!

  2. Materials and methods • Plant material: • 2 maize genotypes (twosamplingtimes) • 13 wheat genotypes • Infection: • Fusariumgraminearum • Fusariumculmorum • Fusariumverticillioides • Samplepreparation: • 500 – 1000 mg fromeachsample • microwavedigestioninnitricacid and hydrogenperoxide • dilutionto 200 - 400 X • Measurement: • Microelementanalysis (Mn, Fe, Co, Ni, Cu, Zn, As, Se, Mo, Cd, Pb) by ICP-MS device Two countries, one goal, joint success!

  3. The advantages of an ICP-MS • More than 1000 samples in one week (up to 250 samples/day) • More than 15000 data from the analysis • Lot of information in a few seconds (less than a second per element) • User-friendly software (PlasmaLab), which makes the data analysis easier and faster Two countries, one goal, joint success!

  4. Microelement contents in thetwo maize genotypes GK-Sarolta GK-Boglár Two countries, one goal, joint success!

  5. Mn content in maize genotypes Two countries, one goal, joint success!

  6. Fe content in maize genotypes Two countries, one goal, joint success!

  7. Co content in maize genotypes Two countries, one goal, joint success!

  8. Ni content in maize genotypes Two countries, one goal, joint success!

  9. Cu content in maize genotypes Two countries, one goal, joint success!

  10. Zn content in maize genotypes Two countries, one goal, joint success!

  11. As content in maize genotypes Two countries, one goal, joint success!

  12. Se content in maize genotypes Two countries, one goal, joint success!

  13. Mo content in maize genotypes Two countries, one goal, joint success!

  14. Cd content in maize genotypes Two countries, one goal, joint success!

  15. Pb content in maize genotypes Two countries, one goal, joint success!

  16. Microelement contents in 13 different wheat genotypes • Manastur - TM • Sanandrei - TM • Faget-TM • Pietroasa- TM • Lovrin -TM • Aldesti -TM • Dudestii Vechi -TM • Horea - Ar • Siria - Ar • Recas - Ar • Semlac - Ar • Paulis - Ar • Barzava - Ar Two countries, one goal, joint success!

  17. Mn content in different wheat genotypes Two countries, one goal, joint success!

  18. Fe content in different wheat genotypes Two countries, one goal, joint success!

  19. Co content in different wheat genotypes Two countries, one goal, joint success!

  20. Ni content in different wheat genotypes Two countries, one goal, joint success!

  21. Cu content in different wheat genotypes Two countries, one goal, joint success!

  22. Zn content in different wheat genotypes Two countries, one goal, joint success!

  23. As content in different wheat genotypes Two countries, one goal, joint success!

  24. Se content in different wheat genotypes Two countries, one goal, joint success!

  25. Mo content in different wheat genotypes Two countries, one goal, joint success!

  26. Cd content in different wheat genotypes Two countries, one goal, joint success!

  27. Pb content in different wheat genotypes Two countries, one goal, joint success!

  28. Pb content in different wheat genotypes Two countries, one goal, joint success!

  29. Summary Maizegenotypes: • Infections by all Fusarium strains increased the concentrations of microelements (Mn, Fe, Ni, Cu) and toxic metals (Cd, Pb) in the grains Wheatgenotypes: • Mn and Ni concentrationwerehighinBarzava-Ar • Fe, Co, Ascontentwerehigherinthechaffcomparedtograinsinallgenotypes • Inthechaff of Horea-Arhigh Pb and Asconcentrationsweremeasured protectionforthegrain Two countries, one goal, joint success!

  30. THANK YOU FOR YOUR ATTENTION! Two countries, one goal, joint success!

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