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FLT. Given a photosynthesis role play I will be able to model the steps of the light reaction and calvin cycle through manipulates and partner. Light Reaction.
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FLT Given a photosynthesis role play I will be able to model the steps of the light reaction and calvin cycle through manipulates and partner
Everyone will take part in our photosynthesis theatherPart 1--Actors1) Photosystem II2) ETC3) Last ETC4) Photosystem I5) ATP synthase 6) Sun 7) Stroma 8) Water 9) ADP (also in part III)10) NADP+(also in part III)11) Thylakoid space
Roles for Part I • Sun: Holds flashlight and shines it on PhotosystemsPS II: passes e- cards to last ETCETC: Passes e- cards along line and pumps H+ cards over shoulder to thylakoid spacePS I: Passes e- cards to LAST ETCWATER: Passes e- cards to PS IITHYLAKOID SPACE: Receives H+ cards from ETC and passes H+ cards to ATP synthaseATP SYNTHASE: receives H+ cards from thylakoid space and passes them to stroma; holds P cards and attaches them to ADP.LAST ETC: collects all e- cards as they come down line, receives H+ from stroma and gives 2 e- cards and attaches an H+ to NADP+ • ACT OUT ETC first. Where is this happening? What are the jobs? Where do electrons come from to replace those lost from PS II? Where do ATP and NADPH go next?
Arrange yourselves • Students wear appropriate name tags and line up in correct position. • Photosystem II, ETC, ETC, ETC, Photosystem I, ETC, ATP synthase stand in a lineSun stands to side of PS II.Stroma stands in front of line.Water stands next to thylakoid space.ADP stands near ATP synthaseNADP+ stands near ATP synthase and last ETC
Actors—Calvin Cycle 5 Actors:1 CO2 ATP and NADPH from light reactions1 Stroma 1 RuBP • Stroma, “Hi, I’m stroma. The Calvin Cycle happens around me” • CO2 enters and high fives stroma, “Hi, I’m CO2” (circle stroma) • RuBP enters and high fives stroma “Hi, I’m RuBP” (circle stroma CO2 and RuBP link arms . Both say “We are joined by RUBISCO. We are now a 6 carbon molecule. Too bad we are unstable. Let’s become two 3 carbon molecules. Just call us PGA.” • ATP removes its P and says “I am now ADP and are low energy. Back to the light reaction.” • NADPH removes its H+ and passes it and 2 e- cards to STROMA. “I am now NADP+ and are missing electrons. Back to the light reaction.” STROMA takes 2 e- and H+ from NADPH, and energy and P from ATP. • STROMA yells ”I need 2 more CO2 and RuBP so I can make G3P and recycle RuBP”
Part II • SPLITTING WATER:Assign 8 students to be water molecules in THYLAKOID SPACE.Each starts wearing an O card with 2 H's attached and holds 2 e- cards. • As water is split, the H+ cards are detached and are passed to the THYLAKOID SPACE.The 2 e- cards pass to PS II, and the leftover O holds hands and joins with another O to become O2 and leaves the chloroplast (room).
5C 1C 3C 3C CO2 C 5C C 3 ATP C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C 3 ADP 3C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C 6C = = C C C 6 ATP 6 NADPH H H H H H H | | | | | | – – C C C 6 NADP 6 ADP C C Calvin cycle C 1. Carbon fixation 3. Regenerationof RuBP RuBP RuBisCo ribulose bisphosphate starch,sucrose,cellulose& more ribulose bisphosphate carboxylase used to makeglucose glyceraldehyde-3-P PGA G3P phosphoglycerate 2. Reduction
1C 5C 3C CO2 RuBP 5C 6C unstable intermediate ATP ADP 3C ATP PGA ADP G3P 3C NADPH NADP Calvin cycle when CO2 is abundant RuBisCo G3P to make glucose C3 plants
ETC: Passes e- cards along line and pumps H+ cards over shoulder to thylakoid space
THYLAKOID SPACE: Receives H+ cards from ETC and passes H+ cards to ATP synthase
ATP SYNTHASE: receives H+ cards from thylakoid space and passes them to stroma; holds P cards and attaches them to ADP.
LAST ETC: collects all e- cards as they come down line, receives H+ from stroma and gives 2 e- cards and attaches an H+ to NADP+
ADENOSINE P P
ADENOSINE P P
ADENOSINE P P
ADENOSINE P P
ADENOSINE P P
P P P P P P
CO2 CO2 CO2 CO2 CO2 CO2
CO2 CO2 CO2 CO2 CO2 CO2
CO2 CO2 CO2 CO2 CO2 CO2
H+ H+ H+ H+ H+ H+
H+ H+ H+ H+ H+ H+
H+ H+ H+ H+ H+ H+