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FLT

FLT. Given a photosynthesis role play I will be able to model the steps of the light reaction and calvin cycle through manipulates and partner. Light Reaction.

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FLT

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  1. FLT Given a photosynthesis role play I will be able to model the steps of the light reaction and calvin cycle through manipulates and partner

  2. Light Reaction

  3. Everyone will take part in our photosynthesis theatherPart 1--Actors1) Photosystem II2) ETC3) Last ETC4) Photosystem I5) ATP synthase 6) Sun 7) Stroma 8) Water 9) ADP (also in part III)10) NADP+(also in part III)11) Thylakoid space

  4. Roles for Part I • Sun: Holds flashlight and shines it on PhotosystemsPS II: passes e- cards to last ETCETC: Passes e- cards along line and pumps H+ cards over shoulder to thylakoid spacePS I: Passes e- cards to LAST ETCWATER: Passes e- cards to PS IITHYLAKOID SPACE:  Receives H+ cards from ETC and passes H+ cards to ATP synthaseATP SYNTHASE:  receives H+ cards from thylakoid space  and passes them to stroma; holds P cards and attaches them to ADP.LAST ETC: collects all e- cards as they come down line, receives H+ from stroma and gives 2 e- cards and attaches an H+ to NADP+ • ACT OUT ETC first. Where is this happening? What are the jobs? Where do electrons come from to replace those lost from PS II? Where do ATP and NADPH go next?

  5. Arrange yourselves • Students wear appropriate name tags and line up in correct position. • Photosystem II, ETC, ETC, ETC, Photosystem I, ETC, ATP synthase stand in a lineSun stands to side of PS II.Stroma stands in front of line.Water stands next to thylakoid space.ADP stands near ATP synthaseNADP+ stands near ATP synthase and last ETC

  6. Light Reaction

  7. Actors—Calvin Cycle 5 Actors:1 CO2 ATP and NADPH from light reactions1 Stroma 1 RuBP • Stroma, “Hi, I’m stroma. The Calvin Cycle happens around me” • CO2 enters and high fives stroma, “Hi, I’m CO2” (circle stroma) • RuBP enters and high fives stroma “Hi, I’m RuBP” (circle stroma CO2 and RuBP link arms . Both say “We are joined by RUBISCO. We are now a 6 carbon molecule. Too bad we are unstable. Let’s become two 3 carbon molecules. Just call us PGA.” • ATP removes its P and says “I am now ADP and are low energy. Back to the light reaction.” • NADPH removes its H+ and passes it and 2 e- cards to STROMA. “I am now NADP+ and are missing electrons. Back to the light reaction.” STROMA takes 2 e- and H+ from NADPH, and energy and P from ATP. • STROMA yells ”I need 2 more CO2 and RuBP so I can make G3P and recycle RuBP”

  8. Part II • SPLITTING WATER:Assign 8 students to be water molecules in THYLAKOID SPACE.Each starts wearing an O card with 2 H's attached and holds 2 e- cards. • As water is split, the H+ cards are detached and are passed to the THYLAKOID SPACE.The 2 e- cards pass to PS II, and the leftover O holds hands and joins with another O to become O2 and leaves the chloroplast (room).

  9. Calvin Cycle

  10. 5C 1C 3C 3C CO2 C 5C C 3 ATP C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C 3 ADP 3C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C 6C = = C C C 6 ATP 6 NADPH H H H H H H | | | | | | – – C C C 6 NADP 6 ADP C C Calvin cycle C 1. Carbon fixation 3. Regenerationof RuBP RuBP RuBisCo ribulose bisphosphate starch,sucrose,cellulose& more ribulose bisphosphate carboxylase used to makeglucose glyceraldehyde-3-P PGA G3P phosphoglycerate 2. Reduction

  11. 1C 5C 3C CO2 RuBP 5C 6C unstable intermediate ATP ADP 3C ATP PGA ADP G3P 3C NADPH NADP Calvin cycle when CO2 is abundant RuBisCo G3P to make glucose C3 plants

  12. Sun: Holds flashlight and shines it on Photosystems

  13. PS II: passes e- cards to last ETC

  14. ETC: Passes e- cards along line and pumps H+ cards over shoulder to thylakoid space

  15. PS I: Passes e- cards to LAST ETC

  16. WATER: Passes e- cards to PS II

  17. THYLAKOID SPACE:  Receives H+ cards from ETC and passes H+ cards to ATP synthase

  18. ATP SYNTHASE:  receives H+ cards from thylakoid space  and passes them to stroma; holds P cards and attaches them to ADP.

  19. LAST ETC: collects all e- cards as they come down line, receives H+ from stroma and gives 2 e- cards and attaches an H+ to NADP+

  20. ATPSYNTHASE

  21. ADENOSINE P P

  22. ADENOSINE P P

  23. ADENOSINE P P

  24. ADENOSINE P P

  25. ADENOSINE P P

  26. P P P P P P

  27. ETC

  28. ETC

  29. ETC

  30. ETC

  31. CO2 CO2 CO2 CO2 CO2 CO2

  32. CO2 CO2 CO2 CO2 CO2 CO2

  33. CO2 CO2 CO2 CO2 CO2 CO2

  34. WATER(H2O)

  35. NADP +

  36. NADP +

  37. NADP +

  38. NADP +

  39. NADP +

  40. H+ H+ H+ H+ H+ H+

  41. H+ H+ H+ H+ H+ H+

  42. H+ H+ H+ H+ H+ H+

  43. O

  44. PHOTOSYSTEM I

  45. PHOTOSYSTEM II

  46. CALVIN CYCLE

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