Whole Lotta Shakin’ ; Dawn A. Bonnell, University of Pennsylvania, DMR 0425780

1. Whole Lotta Shakin’ ;Dawn A. Bonnell, University of Pennsylvania, DMR 0425780 Far left: Two color fluorescence image of a Drosophila S2 cell with microtubules shown in red and peroxisomes in green. Left: individual frames showing motions. Below: evidence for correlated motion of distant cargos. Simultaneously imaging both microtubule bundles, and their cellular cargos, showed that microtubule sliding is an important part of cellular transport. IM Kulic and PC Nelson, Europhys Lett 2007; IM Kulić, AEX Brown, H Kim, C Kural, B Blehm, PR Selvin, PC Nelson and VI Gelfand, The role of microtubule movement in bidirectional organelle transport. PNAS 2008.

2. Whole Lotta Shakin’ Broader Impact Dawn A. Bonnell, University of Pennsylvania, DMR 0425780 We have exquisite information about the static structures of cellular components, but still little understanding of how they orchestrate their many dynamic functions. The understanding now beginning to emerge will influence cell biology over the coming years. Recently developed imaging technology allows us to look inside living cells with unprecedented spatial and time resolution (nanometers and milliseconds). Extending this method to two colors allowed us to perceive previously hidden relations between cell components. For example, our work documented correlation between the motions of distant call cargos, indicating that they were both attached to the same active structural element (a microtubule). Similarly, relative motion of two cargos was correlated to the unbundling of their common microtubule track. Right: Various processes and arrangements of microtubules emanating from a cell’s nucleus, and observed in our work. IM Kulić, AEX Brown, H Kim, C Kural, B Blehm, PR Selvin, PC Nelson and VI Gelfand, The role of microtubule movement in bidirectional organelle transport. PNAS 2008.