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Photosynthesis. Light energy. CO 2. H 2 O. C 6 H 12 O 6. O 2. 6. +. 6. +. 6. Carbon dioxide. Water. Glucose. Oxygen gas. PHOTOSYNTHESIS. 0. Photosynthesis. 0. Human demand for energy Fossil fuel supplies? Energy plantations Biomass energy. Carbon and Energy.
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Light energy CO2 H2O C6H12O6 O2 6 + 6 + 6 Carbon dioxide Water Glucose Oxygen gas PHOTOSYNTHESIS 0 Photosynthesis
0 • Human demand for energy • Fossil fuel supplies? • Energy plantations • Biomass energy
Carbon and Energy • Photoautotrophs • C source? • Energy source? • Heterotrophs • C source? • Energy source?
Figure 7.1A–D Photoautotrophs
Mesophyll Cell Leaf Cross Section Leaf Mesophyll LM 2,600 Chloroplast Vein Stoma O2 CO2 Chloroplast Outer membrane TEM 9,750 Inner membrane Stroma Intermembrane space Stroma Grana Thylakoid Granum Thylakoid space Where does PS happen? • Green parts… • Chloroplasts (stroma and thylakoids) • Stomata
Where does O2 come from? • By splitting water
Reduction 6 O2 6 CO2 6 H2O C6H12O6 Oxidation Oxidation 6 H2O C6H12O6 6 CO2 6 O2 Reduction 0 Just like respiration? • Redox processes
Photosynthesis Energy-storing Releases O2 Requires CO2 Aerobic Respiration Energy-releasing Requires O2 Releases CO2 Linked Processes
H2O CO2 Chloroplast Light NADP+ ADP + P CALVIN CYCLE (in stroma) LIGHT REACTIONS (in thylakoids) ATP Electrons NADPH O Sugar 0 Overview 2 stages • The light reactions… • Energy capture • produce O2 • The Calvin cycle • Uses CO2 • Makes sugar • The Coenzymes • ATP • NADPH
Increasing energy 1 nm 1 m 10–5 nm 10–3 nm 103 nm 106 nm 103 m Micro- waves Radio waves Gamma rays X-rays UV Infrared Light Reflected light Visible light 400 500 600 700 750 380 Wavelength (nm) Absorbed light Chloroplast 650 nm Transmitted light Visible Light
Pigments Colors? • Wavelengths not absorbed • Chlorophylls a and b • Carotenoids
Chlorophylls chlorophyll b chlorophyll a Wavelength absorption (%) Wavelength (nanometers)
Photons • Packets of light energy • Shortest wavelength (blue-violet light) = highest energy
Photosystem Reaction center Primary electron acceptor Light-harvesting complexes Photon Excited state To electron transport chain e– Heat Energy of electron e– Photon Thylakoid membrane Photon (fluorescence) Ground state Pigment molecules Chlorophyll molecule Transfer of energy Chlorophyll a molecule 0 Photons and Photosystems Strike chlorophyll in photosystem Excite an electron Figure 7.7B, C
Photosystem Reaction center Primary electron acceptor Light-harvesting complexes Photon Excited state To electron transport chain e– Heat Energy of electron e– Photon Thylakoid membrane Photon (fluorescence) Ground state Pigment molecules Chlorophyll molecule Transfer of energy Chlorophyll a molecule • Photon energy transfer to reaction-center • Excites electron • Which is taken by the primary electron acceptor • Which leads to the ETC
Photosystem: Harvester Pigments • When excited by light energy:
Photon Photon Photosystem II Photosystem I + H+ NADPH NADP+ Stroma 1 6 e– 2 e– Thylakoid membrane 4 5 P700 1 P680 2 Thylakoid space 3 Electron transport chain Provides energy for synthesis of by chemiosmosis ATP H2O O2 + 2 H+ Electron Transfer Chain • Uses electrons from reaction center • Powers H+ pump to produce ATP • Produces NADPH
e– ATP e– e– NADPH e– e– e– Mill makes ATP Photon e– Photon Figure 7.8B Photosystem II Photosystem I Electron Transfer Chain • Electrons move from photosystem II to I • Make ATP • Electrons from photosystem I • Reduce NADP+ to NADPH
Chloroplast Stroma (low H+ concentration) H+ H+ Light Light ADP + P ATP H+ NADP+ H+ NADPH + H+ Thylakoid membrane H+ H+ H2O 1 H+ H+ H+ H+ + O2 H+ 2 H+ H+ 2 H+ Photosystem II Electron transport chain Photosystem I ATP synthase H+ Thylakoid space (high H+ concentration) ATP Production
Light-Independent Reactions • They put the “synthesis” in photosynthesis • Calvin-Benson cycle • In stroma
Overall reactants CO2 ATP NADPH Overall products Glucose ADP NADP+ Calvin-Benson Cycle Cyclic! RuBP is regenerated
C3 Plants • 1st stable molecule is 3C PGA • C3 plants: tomatoes, petunias, roses, daisies, avocados
C3 Plants • Hot, dry days what happens? • Inside leaf? • O2 increases • CO2 drops • PS rate?
C4 Pathway • CO2 miner • 4C oxaloacetate forms in bundle sheath cells • Grasses
CAM Plants • Opens stomata at night • Forms 4C compound • Release CO2 • Succulents and cacti • Slow growing
Greenhouse Effect? • What is the role of PS in global warming?
Some heat energy escapes into space Sunlight ATMOSPHERE Radiant heat trapped by CO2 and other gases Greenhouse Effect • Excess CO2 in the atmosphere
Question of the Day Scientists at Stanford University conducted a study on California grasslands. They looked at the effects of increased levels of CO2 , soil nitrogen, and temperature (modeling our future) on plant growth. What did they find?