SureSelect™ Decision Tree Focus your next-gen sequencing on DNA that matters

1. SureSelect™ Decision TreeFocus your next-gen sequencing on DNA that matters XT

2. SureSelectTM Decision Tree All Exon 50Mb All Exon v1-38Mb Custom Capture All Exon v2-44Mb Custom Capture Kinome Click Here Kinome X Chromosome What do you want to capture? Non Human Non Human Human Human DNA RNA Whole Exome Mouse Exome Custom Capture Custom Capture

3. SureSelect All Exon KitsWhole Exome in one capture! All Exon 50Mb Performance • Crack the Codes of Mendelian and other Diseases • Less sequencing to results • Up to 20x more samples per run – compared to Whole Genome Sequencing • Most comprehensive coverage in the market Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow

4. SureSelectHuman All Exon Kits Comprehensive coverage • Human All Exon kits can be customized (PLUS) with up to 6.8Mb additional custom content • Human All Exon kits can be multiplexed on SOLiD4 and HiSeq2000 Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow

5. SureSelect All Exon 50Mb • The PLUS option allows addition of up to 6.8Mb of custom content using the eArray tool • The XT option includes also kits for gDNA prep and library prep Click to go back to the decision tree Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow

6. SureSelect All Exon v1 Kits – 38Mb • The PLUS option allows addition of up to 6.8Mb of custom content using the eArray tool • The XT option includes also kits for gDNA prep and library prep Click to go back to the decision tree Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow

7. SureSelect All Exon v2 Kits – 44Mb • The PLUS option allows addition of up to 6.8Mb of custom content using the eArray tool • The XT option includes also kits for gDNA prep and library prep Click to go back to the decision tree Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow

8. SureSelect X-Chromosome • The XT option includes also kits for gDNA prep and library prep Click to go back to the decision tree Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow

9. Definition of SureSelect Human Kinome Kit: 3.2Mb (incl. UTRs): • 518 putative kinases • 12 PI3K domain-containing genes • 13 diglyceridekinases • 6 PI3K regulatory components • 9 inositol polyphosphate Kinases • 9 PIP4/PIP5 Kinases • 28 genes frequently mutated in human cancer • 19 genes specifically known to be mutated in breast cancer • 612 genes total SureSelectKinomeDiscovery and Profiling of Biomarkers for Disease and Drug Response G. Manning et al Science (2002) Original content defined by Prof. René Bernards – NKI Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow

10. SureSelect Human Kinome Kit – DNA • The XT option includes also kits for gDNA prep and library prep Click to go back to the decision tree Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow

11. SureSelect Custom Kits • Capture any custom genomic regions (introns, exons, UTRs, regulatory, etc.) • Ideal for biomarkers discovery and profiling (e.g. cancer) • Ideal for custom SNP follow-up • Ideal for characterization of large sample cohorts • Only pay what you capture, scalable from 0.2 to 6.8Mb (sweet spot for 3rd Gen Seq) • <0.2 Mb • 0.2 – 0.5Mb • 0.5 – 1.5Mb • 1.5 – 3Mb • 3 – 6.8Mb • COMING SOON! – Custom Kits up to 34Mb IMAGE: Nature Immunology Volume: 11, Pages: 197–206 (2010) Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow

12. SureSelect Custom Kits – DNA Click to go back to the decision tree Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow

13. Expanding Exome Design to Animal Models Exon definition derived from Ensembl + RefSeq Designed against mm9 reference from UCSC Number of genes covered: 24,306 Number of exons: 221,784 Total size of design: 49.6 MB Mouse All Exon Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow

14. SureSelect Mouse All Exon Kits • The XT option includes also kits for gDNA prep and library prep Click to go back to the decision tree Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow

15. Performance of SureSelect RNA Target Enrichment Gene Expression Levels Enriched library R2=0.885 Unenriched library High correlation to unenriched RNA library = enrichment process doesn’t create any bias – reliable gene expression results! Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow

16. Performance of SureSelect RNA Target Enrichment Gene Expression Levels Enriched Library 1 R2=0.998 Enriched library 2 High Reproducible process = reliable gene expression results Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow

17. SureSelect Custom Kits - RNA Click to go back to the decision tree Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow

18. SureSelect RNA Capture Human Kinome Kit Click to go back to the decision tree Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow

19. All Exon v2 (44Mb) SureSelectTM Decision Tree All Exon 50Mb All Exon v1 (38Mb) Custom Capture Custom Capture Kinome Kinome X Chromosome What do you want to capture? Non Human Non Human Human Human RNA DNA Whole Exome Mouse Exome Custom Capture Custom Capture