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Trichomoniasis

Trichomoniasis. Field Perspectives Jeremy VanBoening, DVM. Trichomoniasis Involvement. Clinical Practice Nebraska Cattlemen-NCBA Nebraska Veterinary Medical Association . Sample Collection. Texas A&M Trich Sample Collection Video.

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Trichomoniasis

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  1. Trichomoniasis Field Perspectives Jeremy VanBoening, DVM

  2. Trichomoniasis Involvement • Clinical Practice • Nebraska Cattlemen-NCBA • Nebraska Veterinary Medical Association

  3. Sample Collection Texas A&M Trich Sample Collection Video

  4. Sample Collection ChallengesSurvey of 10 D-Labs 10-14-2013

  5. Summary of PCR Related Questions

  6. Sample Collection Challenges Restraint and Safety

  7. Is this safe??

  8. What Collection Method • preputial scraping with pipette • preputial scraping using thrichit • saline rinse • swap or scraping of the penis NO BLOOD for Either PCR or Culture

  9. Sample Collection Challenges Cleanliness • Field Collection Area • Scissors (Disinfect or Not and with what?) • Access to saline for rinsing

  10. Dirty Samples • Rarely if Ever Return Positive • Recent Study Hustun, etal. MSU • 20 Samples w/ bacteria injected at increasing #’s • 20 Samples clean (Only T. Foetus Added) • 0 of 20 with bacteria ever recorded as positive • 20 of 20 w/out bacteria had at least one positive recording

  11. Sample Collection ChallengesProper Collection Media

  12. Sample Preparation • Depends on the D-Lab!! • Collection Medium (In-Pouch, Tubes, Saline) • Incubate or Not • Depends on D-Lab, PCR or Culture, • How should the sample be packaged? • On Ice, Room Temperature, Or Depends on the Weather

  13. Transportation Issues • Rural Access to Timely Reliable Transportation • How to package and send samples? • How long can they be in transit and at what temperature? • Sensitivity and Specificity go down for both culture and PCR Hustunetal MSU

  14. How Do We Get Better???? Standardization • Adopt the Best Diagnostic Testing Available (qPCR) Using Chemical-Lysis and IAC. • Promote SOP’s for Sampling, Sample Prep, and Shipping • Increased Veterinarian/Producer Awareness • Standardized Regulations

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