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Algal Control By Phosphate Restriction

Algal Control By Phosphate Restriction. Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate. Newport Back Bay is nearby and may also be contaminated with the toxic algae. Eastlake (Yorba Linda) is the control lake and is not being treated.

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Algal Control By Phosphate Restriction

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  1. Algal Control By Phosphate Restriction • Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate. • Newport Back Bay is nearby and may also be contaminated with the toxic algae. • Eastlake (Yorba Linda) is the control lake and is not being treated.

  2. Mason Lake Sampling Site

  3. Toxic Algae in Mason Lake

  4. Visibility: 4cm

  5. High phosphate levels are from many water fowl

  6. Toxin-Producing Microcystis sp.

  7. Mason Lake: One Mile From Newport Back Bay Estuary

  8. Newport Back Bay

  9. Urban runoff into the bay Bay

  10. Storm drains with Phosphate-rich water from urban run-off

  11. Phosphate-rich water dumping into the Bay

  12. Upper Newport Bay • Top birding site • Home to 200 species of birds • 30,000 migrating birds per day • Audubon Society/F&G conducts a monthly bird count

  13. Endangered Species at Newport Back Bay: California Least Tern

  14. Light-Footed Clapper Rail

  15. Brown Pelican

  16. Peregrine Falcon

  17. Belding’s Savannah Sparrow

  18. Coastal California Gnatcatcher

  19. East Lake (Yorba Linda) Sampling Site

  20. East Lake: Yorba Linda, CA

  21. Algae is creating a foamy surface scum

  22. A Diatom called Navicula is causing the foam

  23. Sample Analysis • Algal Assay • Species ID • Cell count • Chlorophyll Assay • Flow Cytometry • Species populations • Phosphate Assay

  24. Algal Assay

  25. Eastlake: Diatoms

  26. Mason Lake: Cyanobacteria

  27. Flow Cytometry is used to count algal cells and separate them by species

  28. Flow Cytometry is used to count algal cells and separate them by species

  29. Chlorophyll Assay Filter the water to capture the algae on the filter paper

  30. Mason Lake Eastlake

  31. Grind up filter paper with acetone to release chlorophyll

  32. After Centrifuge

  33. Filter algae out of water, add a reagent that turns blue when it contacts phosphate Phosphate Assay

  34. Mason Lake Eastlake Filters with algae left on them

  35. The deeper the blue color, the more phosphate is present.

  36. A dilution series with phosphate standard

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